iPS Cells: Open Source Resource & Training Course
An Open Source National Resource for iPS Cells
Recently published induced pluripotent stem (iPS) cells consist of reprogrammed skin or blood cells obtained from normal human volunteers or from individuals with a variety of diseases. The reprogrammed cells from a large CTSA-sponsored iPS cell bank are now available free of charge to academic and non-profit investigators as part of an “Open Source Biology” approach to research developed by the CTSI in collaboration with the Center for Regenerative Medicine (CReM) of BU and BMC. Those interested in obtaining a vial of normal or disease-specific iPS cells for disease modeling or therapeutic screens, please contact, Dr. Darrell Kotton, CReM Director (dkotton@bu.edu), or click the link below for Open Source information on the CReM’s CTSA-supported, banked lines and free downloadable protocols: www.bumc.bu.edu/stemcells.
For those interested in understanding the reprogramming methods used to derive the iPS cells available through this repository, an instructional video is available here: http://www.jove.com/video/4327/generation-human-induced-pluripotent-stem-cells-from-peripheral-blood.
Training Course in Human Induced Pluripotent Stem Cells and Their Differentiation into Endoderm and Lung-Progeny
APRIL 4th-8th, 2016
Boston University’s Center for Regenerative Medicine
DESCRIPTION:
This five-day course will focus on deriving, maintaining, characterizing and differentiating human induced pluripotent stem cells (hiPSCs). The course is designed for research scientists working or planning to work with human iPSC culture who have prior experience in general cell culture techniques. It will include hands-on training, lectures and demonstrations from leading experts and educators in the field of stem cell biology from the Center for Regenerative Medicine (CReM) at BUMC. Small class size will enable researchers to learn the entire process of reprogramming from somatic cell preparation, to reprogramming methodologies, iPSC identification, isolation and characterization, and current approaches to their directed differentiation into endodermal and lung lineages. Additional topics will include lectures and training in novel gene editing techniques, including the design and use of CRISPR/Cas9 in iPSCs, and development of 3D culture systems. http://www.bu.edu/dbin/stemcells/
REGISTRATION:
To register, please email Marianne James, PhD (mfjames@bu.edu). Space is limited to ten students. Registration fee of $500 includes all course and laboratory materials as well as lunches for five days. Students are responsible for booking their own travel and accommodation; a block of rooms at discounted rate has been reserved at the Hampton Inn adjacent to the course site (see information below). Detailed information about the CReM laboratories, iPSC Core, and links to downloadable iPSC protocols can be found at: http://www.bu.edu/dbin/stemcells.
http://www.bu.edu/dbin/stemcells/files/iPSC%20Training%20Course%20Ad.pdf