Bruker AVANCE 500 Ultrashield NMR Spectrometer

The multifunctional AVANCE 500 is the centerpiece of the core facility.  It is equipped with six probes for unparalleled versatility.  Some of the spectroscopy capabilities are also available in a 500 MHz standard bore NMR spectrometer housed in the Biophysics department.

Probes:

  • Imaging; sample coils: 5, 10, 20, 30mm
  • Micromouse 30mm, with ergonomic-design cradle
  • Narrow bore 5mm triple resonance inverse solutions NMR
  • Narrow bore 10mm broadband solutions NMR
  • Narrow bore 20mm broadband solutions NMR
  • Wide bore 7mm solids MASS-NMR

Peripheral:

  • Surgivet Anesthesia machine for isoflurane inhalation anesthesia
  • SA nstrumets 2500 small animal monitoring unit for continuous ECG, respiration, and temperature monitoring
  • BSL 2 wet lab

Computing:

  • Linux workstation (new in Jan 2103)-spectrometer operation, data analysis
  • External workstation- with advanced graphics, data analysis
  • Dell laptop- dedicated to animal monitoring, storing of monitor traces

Capabilities:

  • In vivo imaging of mice
  • In vivo spectroscopy of mice
  • Ex vivo tissue microscopy
  • High resolution/solution NMR including high sensitivity analysis and 2D and 3D experiments for protein structure
  • MASS-NMR

Bruker EMX300 EPR Spectrometer

  • X-band (10 GHz) microwave source with high sensitivity chamber
  • L-band (1 GHz) microwave source with resonance chamber

Capabilities:

  • Analysis or detection of tissue free radicals.
  • Can detect 10-10 moles spin.

11.7T MRI upgrade

January 2013 saw the completion of a 3 month upgrade of the 11.7T instrument, including training with the new programming and new acquisition capabilities. The upgrades to the current state of the art (all-digital Avance console with the Bruker Paravision 5/6 data acquisition and analysis) will afford a multitude of imaging capabilities that were previously unrealized. These improvements will have a significant impact on both ongoing and future projects. The major benefit for most user groups’ projects include significant improvement of in vivo imaging capability, with the availability of state of the art cardiac and respiratory triggering, as well as ultra-fast quantitative MRI capacity.

In addition, the availability of new pulse sequences including ultra-short (UTE) and zero-TE (ZTE), susceptibility weighted imaging, and arterial spin labeling, will yield important advantages. Some research examples include :

(i) improved live mouse vascular imaging with enhanced gating capability,
(ii) capability to elucidate novel mechanisms of atherosclerotic plaque characterization with the availability of UTE and ZTE pulse sequences,
(iii) improved live mouse renal and gastric imaging using improvements in gating technique and ultra-short quantitative MRI capabilities, and
(iv) the capability to study mouse models of Alzheimer’s disease and other neurological disease by using arterial spin labeling and BOLD techniques.