Confocal Microscope Facility

The Confocal Facility provides confocal microscopy services for cell imaging and analysis, as well as assistance in project/experiment planning.  There are currently three confocal microscopes in the room available for use by students, technicians, postdocs and faculty.  Training is mandatory before using the microscopes. If you would like to use the instruments please contact us to schedule training.

Contacts

Locations

Vickery Trinkaus-Randall, PhD
TEL: 617-638-5099
Email: vickery@bu.eduCheryl Chi, MS
TEL: 617-638-4536
Email: cherylchi@gmail.com
BUSM Instructional Building
(L building)
Room L904Housman Medical Research Center
(R Building)
Room R918

INSTRUMENTS and SERVICES

The facility houses 3 Zeiss LSM confocal microscopes acquired from NCRR grants and NIH supplements. The confocal microscopes are housed in R918.

The Zeiss Axiovert 100M microscope has argon laser lines (458 and 488 nm) and 2 HeNe lasers (568 and 633 nm), an LSM 510 electronics control module v1.6 with 3 confocal detection channels for fluorescence or reflected light, and 10,20,25,40 and 63x objectives with DIC sliders for 25 and 40x objectives. Images are captured on a computer with command driven proprietary Zeiss LSM software. This instrument is a workhorse and routinely used for live cell Ca2+ imaging using a flow through system and micromanipulator. The latter are available upon discussion with Dr. Trinkaus-Randall. Standard software includes system configuration, ReUse, Acquisition modes, Auto-Z, crop, ROI scan and bleach, FRET, multitracking to minimize problems of cross-over, image display, analysis, processing and archiving. The physiology and time series programs are present.

The LSM 510 Zeiss Axiovert 200M (v4.0) has 5 argon laser lines, 2 HeNe lasers (568 and 633 nm) and a 405 nm laser. Standard software includes system configuration, ReUse, Acquisition modes, Auto-Z, crop, ROI scan and bleach, FRET, multitracking to minimize problems of cross-over, image display, analysis, processing and archiving. Programs include the physiology, time series using multiple regions, tileware, autofocus and stitchart. The objectives include a 10x dry, 20x dry, 2- 40x (water and oil), and a 63x oil objective. The instrument is equipped with a motorized stage and environmental chamber. Files are saved in the data base format and can be exported using a large number of formats and movies can also be exported. This data can be saved in Excel and exported.

The Zeiss 700 is equipped with solid state lasers and runs on the ZEN software. Standard software includes system configuration, ReUse, Acquisition modes, Auto-Z, crop, ROI scan and bleach, FRET, multitracking to minimize problems of cross-over, analysis, processing and archiving. Programs include the physiology, time series using multiple regions, and tileware. Using the Zeiss Software, one can assess intensity values (ROI), 3-D images, histograms throughout structures and co-localization. This data can be saved in Excel and exported.

Images are only to be removed to a MAC server in the room and then removed from there. A workstation with PC computer includes freeware software for imaging including Image J, NIH Image and Retrac in addition to the full proprietary software of Zeiss for LSM 510 that is available to all users in L904.

Usage

Persons are trained and then evaluated before working independently. Training usually consists of 2-2h sessions. In some cases both parties decide that it is better for the imaging to be performed in an assisted manner.

Sign up may be done by physically coming to L904 or emailing either vickery@bu.edu or cherylchi@gmail.com for time. The key is in L904 and after hours is to be returned to the conference room.

Rates

BUMC Users External Users Hours Available*
Unassisted                  $18/hr
Assisted                       $75/hr
Unassisted                  $100/hr
Assisted                       $140/hr
M-F – 9AM- 6PM

*Additional hours are available for persons who have been approved by Dr. Trinkaus-Randall.