Center Location (with other satellite sites in W-, X-, E-, and R-buildings, X- or Evans Biomedical Research Center): 670 Albany Street, Lab 251, Boston, MA 02118
Real Time PCR Systems (two sets)
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DOM locations:
X730 Bench 5 with instrument manager Reiko Matsui, MD
W624 with instrucment managers Yasuhide Asaumi, MD and Ann Marie Quito
The StepOne™ Real-Time PCR System made by Applied Biosystems is the latest entry-level real-time PCR instrument used to amplify and quantify a targeted DNA or RNA molecule in a sample.
Primer Express Software v3.0
DOM location: 670 Albany Street, Lab 251
Instrument Manager: Lynn Lingyi Deng, PhD
The Primer Express® Software allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications.
Immunohistochemistry (IHC) Facility
NEW!
DOM locations: X-Lab 430
Instrument Manager: Xuemei Zhong, Ph.D., 617-638-7028
More details
With an IntelliPATH FLX system, an automated slide stainer made by BioCare, will assist investigators with process immuno-incubation and color development of tissue slides for immunolabeling. With highly skilled professionals and cutting-edge automated instruments, this facility will assist BUMC researchers to prepare high quality immuno-labeled slides for further histological investigation.
Luminescent Image Analyzer or Gel Imagers (3 sets)
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DOM locations: X-Lab 430, Pulm E-604, and X-Lab 840
Instrument Manager: Isabel Dominguez, Ph.D. , Maria Ramirez, Ph.D. , and Jude Deeney, PhD
The LAS-4000 Luminescent Image Analyzer by FujiFilm captures images of agarose and polyacrylimide gels, Western blot, TLC and small animals. High sensitivity and resolution of in vivo imaging is also possible with small animals such as a mouse.
Nanodrop UV Visible Spectrophotometer
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DOM Location: X-Lab 730
Instrument Manager: Reiko Matsui, MD
The NanoDrop® ND-1000 UV-Vis Spectrophotometer is a new generation of spectrophotometer which enables highly accurate analyses of 1 ul samples for DNA, RNA, protein, pigments, whole uv-visible (220-700nm) spectrum and cell density tests with remarkable reproducibility.
Auto Blood Analyzer HEMAVET 950FS (Fast, Convenient Veterinary Cell Counter)
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DOM Location: 670 Albany Street, Lab 251
Instrument Manager: Michael Boosalis, PhD
The HEMAVET 950FS can perform automated blood counts including; hemoglobin, hematocrit, white blood cells, platelets, differentials, and red blood cell indices for six animals. Parameters include: WBC, NE#, NE%, LY#, LY%, MO#, MO%, EO#, EO%, BA#, BA%, RBC, Hb, HCT, MCV, MCH, MCHC, RDW
plus additional flagging for NRBC#, NRBC%, Platelet Count, MPV
3D cytograms : WBC subpopulations, RBCs, PLTs.
Microplate Reader-Infinite® M1000, Tecan
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DOM Location: X-Lab 810
Instrument Manager: Jude Deeney, Ph.D
The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. The Reader is capable of monitoring UV and visible absorbencies and fluorescence of the samples held in the 96, 396 and other microplates. In addition, the Infinite® M1000 Reader is able to detect the following:
- Fluorescence resonance energy transfer (FRET)
Time resolved fluorescence (TRF)
- Fluorescence intensity top and bottom reading
Flash luminescence
- Glow luminescence
- Dual color luminescence including filter for BRET1 & BRET 2 applications)
- Kinetic studies
The flexibility, sensitivity and speed are features of this newly designed optical instrument.
Extracellular Flux Analyzer or Oxygen Analyzer
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DOM Location: X-Lab 810
Instrument Manager: Yaguang Si, PhD
This software has been updated and the new vrsion is available by Dr. Si.
The XF24 Extracellular Flux Analyzer from Seahorse Bioscience Inc. measures the rate of change of currently dissolved oxygen and pH in the media immediately surrounding living cells cultured in a microplate. It is designed for cell metabolism, drug and regulator studies.
2-Dimentional PAGE Apparatus, Bio-Rad
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DOM Location: 670 Albany Ave., Lab232
Instrument Manager: Wen Guo, MD, PhD
1. PROTEAN IEF Cell
The PROTEAN IEF cell is used for running IPG strips or iso-electric focusing (IEF) polyachrylamide gel electrophoresis (PAGE). The cell can be used to run multiple IEF strips, with temperature control (10–25°C) and gel developing systems.
The PROTEAN IEF cell is a key component of the Expression Proteomics line of products designed to meet the needs of high-throughput labs and occasional users for IEF PAGE.
2. PROTEAN II Gel Electrophoresis Apparatus
The PROTEAN II Apparatus can be used for proteins, DNA and carbohydrates separation either by native PAGE or SDS-PAGE applications with regular sized gels. PROTEAN II Apparatus can also be used for the 2nd dimension of the 2-dimentional PAGE (after IEF PAGE). 
Free Flow Electrophoresis System or FFE-IEF
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DOM Location: 670 Albany Street, Lab 251
Instrument Manager: Lynn Lingyi Deng, PhD
The Free Flow Electrophoresis (FFE) system from DB is used for purification and separation of organelles, protein complexes, protein isoforms and membrane proteins. FFE provides proteomics researchers with a tool to reduce the complexity of samples and enrich low abundant proteins with very high reproducibility. Both IEF and Zone electrophoreses can be performed with this instrument. The samples are kept in liquid phase through the entire run which can be further analyzed by other instrumentations.
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