BU-CMD 16th Annual Symposium
“Molecular Discovery: From Chemical Synthesis to Biological Applications”
Friday, June 23, 2017
Metcalf Center for Science and Engineering, Room 109
590 Commonwealth Avenue, Boston, MA
9:30 am – 5 pm
For more information please see our CMD Symposium site.
Thanks to all who attended the 16th Annual CMD Symposium on June 23! Attendees were treated to fantastic talks from Professors Dirk Trauner, Ben List, Frances Arnold and Robert Langer, followed by a poster session highlighting new research from our students and postdocs.
Special thanks to Center Administrator Lisa Holik for a great job organizing this event, our friends at BUnano for help with setup and Prof. Aaron Beeler for organizing the poster session.
Thanks also to our generous sponsors listed below, without whom this event would not be possible:
- Bristol-Myers Squibb
- BU Translational Research in Biomaterials Training Grant
- Eisai Research Institute
- Interchim Inc.
- Paraza Pharmaceuticals
- Pfizer, Inc.
- Strem Chemicals, Inc.
Check back in the future for news on our next CMD Symposium.
Congratulations to Han Yueh, Qiwen Gao, and Professors John Porco and Aaron Beeler on their recent paper in Bioorganic & Medicinal Chemistry entitled “A photochemical flow reactor for large scale syntheses of aglain and rocaglate natural product analogues”
Abstract: Herein, we report the development of continuous flow photoreactors for large scale ESIPT-mediated [3 +2]-photocycloaddition of 2-(p-methoxyphenyl)-3-hydroxyflavone and cinnamate-derived dipolarophiles. These reactors can be efficiently numbered up to increase throughput two orders of magnitude greater than the corresponding batch reactions.
Congratulations to William Devine, Lauren Brown, and Professor John Porco on their recent paper in Science Translational Medicine entitled “Inhibiting the oncogenic translation program is an effective therapeutic strategy in multiple myeloma.”
Abstract: Multiple myeloma (MM) is a frequently incurable hematological cancer in which overactivity of MYC plays a central role, notably through up-regulation of ribosome biogenesis and translation. To better understand the oncogenic program driven by MYC and investigate its potential as a therapeutic target, we screened a chemically diverse small-molecule library for anti-MM activity. The most potent hits identified were rocaglate scaffold inhibitors of translation initiation. Expression profiling of MM cells revealed reversion of the oncogenic MYC-driven transcriptional program by CMLD010509, the most promising rocaglate. Proteome-wide reversion correlated with selective depletion of short-lived proteins that are key to MM growth and survival, most notably MYC, MDM2, CCND1, MAF, and MCL-1. The efficacy of CMLD010509 in mouse models of MM confirmed the therapeutic relevance of these findings in vivo and supports the feasibility of targeting the oncogenic MYC-driven translation program in MM with rocaglates.
Dr. Karen Allen, Ph.D.
Professor of Chemistry
Dr. Allen brings expertise in the areas of X-Ray crystallography and enzyme structure and function to the CMD.
Dr. Sandor Vajda, Ph.D.
Professor of Biomedical Engineering and Chemistry
Dr. Vajda brings expertise in the areas of computational methods and drug discovery to the CMD.
Dr. Arturo Vegas, Ph.D.
Assistant Professor of Chemistry
Dr. Vegas brings expertise in the areas of drug delivery and targeting to the CMD.
Dr. Adrian Whitty, Ph.D.
Associate Professor of Chemistry
Dr. Whitty brings expertise in the areas of biological chemistry and protein-protein and protein-drug interactions to the CMD.
CMD wishes to Welcome two new members to the team:
David Huang – postdoc
Wenhan Zhang – postdoc
Both will be joining the team in October.
Congratulations to Lauren Brown, Mikayo Hayashi and Professor John Porco on their recent Communication in the European Journal of Organic Chemistry entitled “Asymmetric Dearomatization/ Cyclization Enables Access to Novel Chemotypes.”
Abstract: Enantioenriched, polycyclic compounds were obtained from a simple acylphloroglucinol scaffold. Highly enantioselective dearomatization was accomplished using a Trost ligand palladium(0) complex. A computational DFT model was developed to rationalize observed enantioselectivities and revealed a key reactant-ligand hydrogen bonding interaction. Dearomatized products were used in visible light-mediated photocycloadditions and oxidative free radical cyclizations to obtain novel polycyclic chemotypes including tricyclo[4.3.1.01,4]decan-10-ones, bicyclo[3.2.1]octan-8-ones and highly-substituted cycloheptanones.
The National Institutes of Health (NIH) has awarded a four-year grant to Center for Molecular Discovery researcher Lauren Brown and collaborators Leah Cowen (University of Toronto) and Luke Whitesell (Whitehead Institute for Biomedical Research at MIT) on “Targeting Hsp90 in crytococcal fungal pathogenesis.” The assembled team combines expertise in fungal biology (Cowen), medicinal chemistry (Brown) and pharmacology/experimental therapeutics (Whitesell) with the goal of developing drug-like small-molecule probes for use in studying disease mechanisms of the fungus Cryptococcus.
Invasive Cryptococcus infections pose a grave threat to human health and have enormous economic consequences. Cryptococcal meningitis, the major clinical manifestation of the disease, has a 100% mortatlity rate if left untreated. Even with the best available therapies, mortality rates remain high at 35-40% due to a limited number of drug classes available, and compromised usefulness of these drugs caused by both dose-limiting toxicity and the emergence of high-grade antifungal drug resistance.
In this project, the team will collaborate to develop small molecule chemical compounds to study a critical molecular mechanism that supports both fungal virulence and the onset of drug-resistance. These compounds will also impact clinical care by serving as promising leads for the future development of new, more effective antifungal drugs.
The National Institutes of Health, Institute of General Medical Sciences (NIGMS), has awarded Professor John Porco and coworkers a five year MIRA (R35) grant entitled “Chemical Synthesis of Complex Natural Products for Translational Science.” The Maximizing Investigators’ Research Award (MIRA-R35), an Outstanding Investigator Award, is a grant that provides support for all of the research in an investigator’s laboratory that falls within the mission of NIGMS. Within these bounds, investigators have the freedom to explore new avenues of inquiry that arise during the course of their research. The goals of the MIRA (R35) research program are to continue chemical syntheses of bioactive molecules and expand efforts and capabilities in translational science. The MIRA effort effectively replaces two previous NIGMS-funded RO1 grants (Biomimetic Synthesis of Complex Natural Products (GM-073855) and Chemical Synthesis of Bioactive Flavonoid and Xanthone-Derived Natural Products (GM-099920) which were highly productive and led to 41 publications from 2010 – 2015. As part of the MIRA project, the Porco group will continue development of novel synthetic methodologies for concise entry to bioactive classes of natural products including oxaphenalenones, meroterpenoids, polyprenylated acylphloroglucinols, tetrahydroxanthones, and dimeric chromones. The project will also continue major emphasis on collaborations to study biological properties and mode of action (MoA) of target molecules for ultimate use as pharmacological therapies for human cancers as well as viral and bacterial illnesses.
Congratulations to Dr. William Devine and Professors Aaron Beeler and John Porco on their paper “Fine-tuning of macrophage activation using synthetic rocaglate derivatives” in Nature Scientific Reports, published April, 2016. This work, on novel activity identified for the rocaglates, was done in collaboration with the Beeler group, the CMD and with our collaborator Igor Kramnik, and coworkers, at the BU NEIDL.
Abstract: Drug-resistant bacteria represent a significant global threat. Given the dearth of new antibiotics, host-directed therapies (HDTs) are especially desirable. As IFN-gamma (IFNγ) plays a central role in host resistance to intracellular bacteria, including Mycobacterium tuberculosis, we searched for small molecules to augment the IFNγ response in macrophages. Using an interferon-inducible nuclear protein Ipr1 as a biomarker of macrophage activation, we performed a high-throughput screen and identified molecules that synergized with low concentration of IFNγ. Several active compounds belonged to the flavagline (rocaglate) family. In primary macrophages a subset of rocaglates 1) synergized with low concentrations of IFNγ in stimulating expression of a subset of IFN-inducible genes, including a key regulator of the IFNγ network, Irf1; 2) suppressed the expression of inducible nitric oxide synthase and type I IFN and 3) induced autophagy. These compounds may represent a basis for acrophage-directed therapies that fine-tune macrophage effector functions to combat intracellular pathogens and reduce inflammatory tissue damage. These therapies would be especially relevant to fighting drug-resistant pathogens, where improving host immunity may prove to be the ultimate resource.
(CMD Publications #90)
Professor Porco spoke at The 33rd Herbert C. Brown Lectures in Organic Chemistry, Purdue University, on April 15, 2016. His talk was entitled “Chemical Synthesis and Biological Studies of the Rocaglates and Derivatives.”
Abstract: The plant genus Aglaia produces a number of secondary metabolites including the cyclopenta[b]benzofuran silvestrol. Cyclopenta[b]benzofuran natural products possess potent anticancer properties due to modulation of the activity of the RNA helicase eukaryotic initiation factor 4A (eIF4A), which is involved in loading ribosomes onto mRNA templates during translation initiation, a step frequently deregulated in cancer. In this presentation, we will describe our efforts to synthesize silvestrol and rocaglate analogues using photocycloaddition of 3-hydroxyflavones with various dipolarophiles, and evaluation of the rocaglates produced as inhibitors of eukaryotic protein translation.
Porco – Brown Lect Fig