TitleVeterinary Field Test as Screening Tool for Mastitis and HIV-1 Viral Load in Breastmilk from HIV-Infected Zambian Women
AuthorsDorosko S. M., Thea D. M., Saperstein G., Russell R. M., Paape M. J., Hinckley L. S., Decker W. D., Semrau K., Sinkala M., Kasonde P., Kankasa C., Aldrovandi G. M., Hamer D. H.
PublicationBreastfeed Med. 2007 Oct; 2(3):172-5.
AbstractClinical and subclinical mastitis increase the risk of mother-to-child transmission (MTCT) of HIV-1 through breastfeeding. We hypothesized that a field test for mastitis used for bovine milk, the California Mastitis Test, would detect high cell counts in milk of HIV-infected women. We also investigated whether total milk cell count would positively correlate with viral HIV-1 RNA in the milk of 128 HIV-positive Zambian women. Mean cell counts in each California Mastitis Test scoring category were significantly different (p < 0.01, n = 232). In a subset of 4-month postpartum milk samples tested for HIV-1 RNA, viral RNA levels did not significantly correlate with total cell count (r = 0.166, p = .244). The CMT may serve as a screening tool for mastitis in breastmilk, but total cell count does not correlate with HIV-1 RNA levels. Since both cell-free and cell-associated virus are associated with increased risk of MTCT, investigation of the relationship between total milk cell count and HIV-1 proviral DNA is warranted before a conclusive determination is made regarding use of the CMT as a clinical screening tool to detect cases at high risk for breastmilk transmission.
Related ProjectsZambia Exclusive Breastfeeding Study (ZEBS)