Vadim V

Vadim V. Demidov

Senior Scientist/Principal Investigator

Freelance Science Writer/Editor

Tel.: 617-353-8490 (office)

Fax: 617-353-8501

E-mail: vvd@bu.edu

Dr. Demidov's current research is focused on gene-targeted drugs, DNA diagnostics, peptide nucleic acid (PNA), rolling-circle amplification (RCA), fluorescent proteins, tracking RNA in vivo, RNA modeling and environmental monitoring.

His other interests include scientific journalism, intellectual property/patent priority issues, history of life sciences and related topics.

SOME PREVIOUS EXPERIENCES

Molecular biophysics and photochemistry of nucleic acids

Biological testing of chemical compounds, including mutagens

Thermal and radiation sterilization of drugs and biomedical materials; involvement into the drug composition/formulation studies (post-sterilization analysis)

Drug stability in blood serum and cellular extracts

Ecological and exploration geochemistry of gold

Genetic monitoring of environmental pollutants

Assessment of sci-tech information, including patents

HONORS & AWARDS

1988 Silver medal from the All-Union (USSR) National Exhibition of Economic Achievements in Moscow for the cycle of works on genetic monitoring of environment

1990 Awarded by the rank of Senior Research Scientist (prestigious scientific certification in USSR/Russia) by the USSR Superior Certifying Commission (VAK)

2001 Boston University Provost's Innovation Fund (PIF) Award

2002 Boston University Special Program for Research Initiation Grants (SPRInG) Award

2003-2007 Listed in Marquis's 'Who's Who in America', 'Who's Who in the World', 'Who's Who in Medicine and Healthcare', 'Who's Who in Science and Engineering'

2004 Invited Member of the Reed-Elsevier Exclusive Reception, Boston, MA (Aug 10)

2006 Listed in the Dictionary of International Biography (33rd edition) and in the Inaugural Edition of Outstanding Scientists of the 21st Century, International Biographical Centre, Cambridge, England

2006 International Professional of the Year Award, International Biographical Centre Awards Board

2006 Ten-Year Service Recognition Award, Boston Univ.

EDUCATION

1977 - M.S. in physical-chemical engineering, Department of Molecular and Chemical Physics of Moscow Physical and Technical Institute, USSR

1980 - Ph.D. in biophysics, Institute of Molecular Genetics of USSR Academy of Sciences/Moscow Physical and Technical Institute, USSR

PRIMARY employment

1980-1985 Junior Researcher at the Research Institute for Biological Testing of Chemical Compounds, Moscow

1985-1987 Researcher at the Moscow Institute of Biotechnology

1987-1990 Senior Researcher at the Institute of Mineralogy, Geochemistry and Crystalochemistry of Rare Elements, Moscow

1990-1993 Senior Researcher at the Institute of Molecular Genetics, Moscow

1993 Visiting Assistant Research Professor in the Department of Biology, George Mason University, Fairfax, Virginia

1993-1994 Visiting Research Professor at the Panum Institute, Copenhagen University, Denmark

1994 Senior Research Associate at the Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, Boston, MA

2001 Principal Investigator, Department of Biomedical Engineering, Boston University

TEACHING EXPERIENCE

1991-1992 Lecture course at the Department of Molecular Biophysics, Moscow Physical & Technical Institute

1985-2006 Supervised 3 PhD students, 9 M.S. & 10 B.S. students at Moscow Phys. Tech. Inst. & Boston Univ. (one MS student won the Provost Recognition Award at the BU Graduate Students Posters Presentation, Mar 28, 2006)

2002 Invited member of MS graduate committee, Dept. Biomedical Engineering, Boston University

2004-2005 Assistance to Prof. N.E. Broude in preparing materials for the lab courses BE505 (Molecular Bioengineering I)

2005 Substitute for Prof. N.E. Broude in two lab classes (gel-shift assay in protein-DNA interactions; BE505 Molecular Bioengineering I)

EDITORSHIP

2004 Contributing Editor, Drug Discovery & Development (Reed Elsevier)

2004 Book Editor, Horizon Sci. Press/Horizon Bioscience (Wymondham ,UK)

2004 Invited participant of the Elsevier Editor's Conference (Santa Fe/NM, Oct 30)

2003-2006 Editorial Board Member for Trends in Biotechnology (Elsevier)and Expert Rev. Mol. Diagn. (Future Drugs Ltd.): wrote several editorials and solicited dozen reviews for both these journals

2006 Editorial Board Member for Current Medicinal Chemistry (Bentham Science Publishers Ltd.)

OTHER PROFESSIONAL ACTIVITIES AND SOCIETIES

1982-1992 Freelance reviewer for the abstract journals Molecular Biology, Bioorganic Chemistry and Physics published in Moscow, USSR by the All-Union Institute for Sci-Tech Information (VINITI)

1989-1992 Translator/analyst of excerpts from medicinal books and commercial flyers for Russian publishing and pharmaceutical companies

1990-1992 Participant of the 3 scientific ecological expeditions on peninsulas Kamchatka and Taimyr and Russian Far East

1991-1992 Member of the International working group of experts on planetary protection (chairman: academician M.V. Ivanov)

1992-1993 Member of the Science Board on the problem of gene targeted drugs of Russian Academy of Science (chairman: academician D.G. Knorre)

1995-2006 Peer-reviewer for J. Biomol. Struct. Dyn. , Trends Biotechnol., Drug Discov. Today, Expert Rev. Mol. Diagn. , Med. Sci. Monitor, Chemistry & Biology, Biochemistry, Nucleic Acids Res., Expert Opin. Biol. Ther., Biotechnol. Progress, IUBMB Life, Chem. Technol. Biotechnol., Life Sciences, Oligonucleotides, Current Med. Chem., Journal of Biotechnology and Nature Biotechnology

2002-2006 Proposal assessor for the Science Foundation Ireland, PPP Foundation (UK), U.S. Civilian Res. & Devel. Foundation (CRDF), Kentucky Sci-Eng Foundation (KSEF), Arizona Disease Control Research Commission (ADCRC), Petroleum Research Fund

2000-2003 Book reviewer/News writer for ChemBioChem, Modern Drug Discovery and Drug Discovery Today magazines

2002 Member of the Journal Club panel/Commentaries writer for Trends in Biotechnology

2002 Member of the New York Academy of Sciences

2003 Biotechnology Analyst, Global Prior Art Inc. (Boston)

2004 Member of scholar/consulting program, Round Table Group Inc. (NY)

2006 Society of Chemical Industry (complimentary membership)

Took part in about 20 scientific international conferences/meetings

SUPPLEMENTARY ACTIVITIES AND SOCIETIES

2003 Member of the Commanders Club, Disabled American Veterans (Bronze Leader)

2004 Founding Member of The National Campaign for Tolerance; name "Vadim V. Demidov" is placed on the Wall of Tolerance in Montgomery, AL

2004 Supporting Member, Southern Poverty Law Center (Rosa Park and Morris Dees, co-founders)

2005 Member of The Planetary Society

2005 Founding Sponsor of The Martin Luther King, Jr. National Memorial to be built on the Mall in Washington, D.C.

2006 Supporting Member, Amnesty International

2006 Supporting Member, WGBH Public TV

GRANT AWARDS

1991-1993 Co-PI, Research grant on genetic monitoring of trace metals in the environment from the Russian State Committee (Ministry) of natural recources and environment

2001-2002 PI, Boston University Provost's Innovation Fund

2002-2003 PI, Boston University Special Program for Research Initiation Grants (SPRInG)

2004 PI, Sponsored Research Agreement with Biokit (Spain) on the DNA diagnostics optimization: terminated by PI at the stage of signing the agreement (~$270,000 in direct cost for two years) due to unexpected conflict of interests with the current research

2005-2006 Co-PI, R21 NIH/NHGRI grant "Single Molecule Haplotype Sequencing" (PI: B. Mishra, NYU)

2006 PI, Sponsored Research Agreement with St. Jude Children's Research Hospital (Memphis, TN) on targeted destruction of pathological mammalian cells: $25,000 of seed money (direct cost) for initial proof-of-principle studies

ISSUED PATENTS

1. Frank-Kamenetskii, M.D., Veselkov, A.G. & Demidov, V.V. (1999) Nucleic Acid Clamps. US patent #6,004,750.

2. Frank-Kamenetskii, M. D., Bukanov, N.O., Demidov, V.V. & Kuhn, H. (2001) Methods and compositions pertaining to PD-loops. US patent #6,265,166.

3. Frank-Kamenetskii, M.D., Bukanov, N.O., Demidov, V.V., Kuhn, H. & Broude, N.E. (2003) Methods and compositions pertaining to PD-loops (patent continuation). US patent #6,596,486 B2.

4. Kolotov, B.A., Demidov, V.V., Kashina, L.I. and Minachyova, L.I. (2005) Method for detection of environmentally polluted areas. Russian patent # RU 2,264,636 C2.

SELECTED PUBLICATIONS (~70 papers are referenced in PubMed and ISI Web-of-Science databases)

*highly cited articles (ref. 4: >200 citations, ref. 6: 150 citations; refs. 8, 9, 10 & 19: >50 citations)

1. Demidov, V.V., Potaman, V.N. and Ulmasov, T.N. (1986) Kinetics of the hydrolysis of adenosine-5'-monophosphoric acid (AMP) at high temperatures. Kinetika i Kataliz (Kinetics & Catalysis) 27, 575-579 (491-495 Engl. translation).

2. Babakina, G.S., Demidov, V.V., Isaeva, I.V., Potaman, V.N., Solyanina, I.P., Trofimov, V.I. and Ulmasov, T.N. (1988) Radiation and thermal stability of phosphadene. Khimiko-farmatsevticheskii Zhurnal (Chem. Pharm. J.) 22, 89-93 (73-77 Engl. translation).

3. Demidov, V.V., Kosovets, Yu.G. and Kogan, B.S. (1991) Trace metals determination in microquantities of nucleic acid samples. Nucleic Acids Res. 19, 3155.

*4. Demidov, V.V. , Potaman, V.N., Frank-Kamenetskii, M.D., Egholm, M., Buchardt, O., Sönnichsen, S.H. and Nielsen, P.E. (1994) Stability of peptide nucleic acids in human serum and cellular extracts. Biochem. Pharmacol. 48, 1310-1313.

5. Demidov, V.V., Potaman, V.N., Solyanina, I.P. and Trofimov, V.I. (1995) Radiation and thermal stabilities of adenine nucleotides. Advances Space Res. 15, (3)131-(3)134.

*6. Demidov, V.V., Yavnilovich, M.V., Belotserkovskii, B.P., Frank-Kamenetskii, M.D. and Nielsen, P.E. (1995) Kinetics and mechanism of polyamide (peptide) nucleic acid binding to duplex DNA. Proc. Natl. Acad. Sci. USA 92, 2637-2641.

7. Veselkov, A.G., Demidov, V.V., Frank-Kamenetskii, M.D. and Nielsen, P.E. (1996) PNA as a rare genome-cutter. Nature 379, 214.

*8. Kuhn, H., Demidov, V.V., Frank-Kamenetskii, M.D. and Nielsen, P.E. (1998) Kinetic sequence discrimination of cationic bis-PNAs upon targeting of double-stranded DNA. Nucleic Acids Res. 26(2), 582-587.

*9. Bukanov, N.O., Demidov, V.V., Nielsen, P.E. and Frank-Kamenetskii, M.D. (1998) PD-Loop: a complex of duplex DNA with an oligonucleotide. Proc. Natl. Acad. Sci. USA 95, 5516-5520 (in the news ##3 & 6; see below).

*10. Kuhn, H., Demidov, V.V., Nielsen, P.E. and Frank-Kamenetskii, M.D. (1999) An experimental study of mechanism and specificity of peptide nucleic acid (PNA) binding to duplex DNA. J. Mol. Biol. 286, 1337-1345.

11. Izvolsky, K.I., Demidov, V.V., Nielsen, P.E. and Frank-Kamenetskii, M.D. (2000) Sequence-specific protection of duplex DNA against restriction and methylation enzymes by pseudocomplementary PNAs. Biochemistry 39, 10908-10913 (in the news #1; see below).

12. Kuhn, H., Demidov, V.V. and Frank-Kamenetskii, M.D. (2000) An earring for the double helix: assembly of topological links comprising duplex DNA and a circular oligodeoxynucleotide. J. Biomol. Struct. Dyn., Conversation 11, Issue #2, 221-225 (in the news #2; see below).

13. Demidov, V.V., Bukanov, N.O. and Frank-Kamenetskii, M.D. (2000) Duplex DNA capture. Curr. Issues Mol. Biol. 2(1), 31-35 (cover picture).

14. Demidov, V.V. (2001) Cracking the genomes: J. Craig Venter at his 55th anniversary. Drug Discov. Today 6(20), 1041 (personalia).

15. Demidov, V.V., Broude, N.E., Lavrentyeva-Smolina, I.V., Kuhn, H. and Frank-Kamenetskii, M.D. (2001) An artificial primosome: design, function, and applications. ChemBioChem 2, 133-139 (in the news ##3, 4, & 5; see below).

16. Kuhn, H., Demidov, V.V., Gildea, B.D., Fiandaca, M.J., Coull, J.M. and Frank-Kamenetskii, M.D. (2001) PNA beacons for duplex DNA. Antisense Nucleic Acid Drug Dev. 11, 265-270 (in the news ##7 & 8; see below).

17. Demidov, V.V. (2001) PD-loop technology: PNA openers at work. Expert Rev. Mol. Diagn. 1(3), 343-351 (review article).

18. Demidov, V.V. (2002) Rolling-circle amplification in DNA diagnostics—the power of simplicity. Expert Rev. Mol. Diagn. 2, 542-548 (review article).

*19. Kuhn, H., Demidov, V.V., Coull, J.M., Gildea, B.D., Fiandaca, M.J. and Frank-Kamenetskii, M.D. (2002) Hybridization of DNA and PNA molecular beacons to single-stranded and double-stranded DNA targets. J. Am. Chem. Soc. 124, 1097-1103 (in the news #9; see below).

20. Kuhn, H., Demidov, V.V. and Frank-Kamenetskii, M.D. (2002) Rolling-circle amplification under topological constraints. Nucleic Acids Res. 30, 574-580 (in the news ##10 & 11; see below).

21. Demidov, V.V., Protozanova, E., Izvolsky, K.I., Price, C., Nielsen, P.E. and Frank-Kamenetskii, M.D. (2002) Kinetics and mechanism of the DNA double helix invasion by pseudocomplementary peptide nucleic acids. Proc. Natl. Acad. Sci. USA 99, 5953-5958.

22. Protozanova, E., Demidov, V.V., Soldatenkov, V., Chasovskikh, S. and Frank-Kamenetskii, M.D. (2002) Tailoring the activity of restriction endonuclease PleI by PNA-induced DNA looping. EMBO Rep. 3, 956-961 (cover picture).

23. Demidov, V.V. (2002) PNA comes of age: from infancy to maturity. Drug Discov. Today 7, 153-155 (guest editorial).

24. Demidov, V.V. (2002) Scholar, inventor and businessman: Charles R. Cantor at his 60th anniversary. Drug Discov. Today 7(16), 849-850 (personalia).

25. Demidov, V.V. (2003) PNA and LNA throw light on DNA. Trends Biotechnol. 21, 4-7 (minireview; cover picture).

26. Demidov, V.V. (2003) Golden jubilee of the DNA double helix. Trends Biotechnol. 21, 139-140 (guest editorial).

27. Demidov, V.V. (2003) DNA diagnostics in the fifty-year retrospect: the journey from the double helix and complementarity principle to DNA chips, PNA technology, DNA nanotechnology, real-time PCR and MEMS-based systems. Expert Rev. Mol. Diagn. 3 (2), 89-92 (guest editorial).

28. Demidov, V.V. (2003) 65th birthday of Edwin M. Southern, molecular biology and biotechnology mastermind. Drug Discov. Today 8(15), 666-667 (personalia).

29. Kuhn, H., Hu, Y., Frank-Kamenetskii, M.D. and Demidov, V.V. (2003) Artificial site-specific DNA-nicking system based on common restriction enzymes assisted by PNA openers. Biochemistry 42, 4985-4992 (in the news ##13 & 14; see below).

30. Smolina, I.V. & Demidov, V.V. (2003) Sequence-universal recognition of duplex DNA by oligonucleotides via pseudocomplementarity and helix invasion. Chemistry & Biology 10, 591-595 (in the news #15; see below).

31. He, Y., Panyutin, I.G., Karavanov, A., Demidov, V.V. and Neumann, R.D. (2004) Sequence-specific DNA strand cleavage by ¹¹¹In-labeled peptide nucleic acids. Eur. J. Nucl. Med. Mol. Imaging 31, 837-845 (in the news #16; see below).

32. Kolotov, B.A., Demidov, V.V. and Volkov, S.N. (2004) Chlorophyll status as the indication of environment degradation upon the contamination with heavy metals. Geoekologiya 2, 20-25.

33. Demidov, V.V. & Frank-Kamenetskii, M.D. (2004) Two sides of the coin: affinity and specificity of nucleic acid interactions. Trends Biochem. Sci. 29, 62-71 (review article; cover picture).

34. Kuhn, H., Cherny, D., Demidov, V.V. and Frank-Kamenetskii, M.D. (2004) Inducing and modulating anisotropic DNA bends by pseudocomplementary peptide nucleic acids. Proc. Natl. Acad. Sci. USA 101, 7548-7553.

35. Smolina, I.V., Demidov, V.V., Cantor, C.R. and Broude, N.E. (2004) Real-time monitoring of branched rolling-circle DNA amplification with peptide nucleic acid beacon. Anal. Biochem. 335, 326-329.

36. Kolotov, B.A., Demidov, V.V. and Volkov, S.N. (2004) Chlorophyll status as the indication of environment degradation upon the contamination with heavy metals. Geoekologiya 2, 20-25.

37. Smolina, I.V., Demidov, V.V., Soldatenkov, V.A., Chasovskikh, S.G. and Frank-Kamenetskii, M.D. (2005) End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes. Nucleic Acids Res. 33(17), e146. [corrigendum in Nucleic Acids Res. 33(19), 6459]

38. Smolina, I.V., Cherny, D.I., Nietupski, R.M., Beals, T., Smith, J.H., Lane, D.J., Broude, N.E. and Demidov, V.V. (2005) High-density fluorescently labeled rolling-circle amplicons for DNA diagnostics. Anal. Biochem. 347, 152-155.

39. Demidov, V.V. (2005) Ten years of rolling the minicircles: RCA assays in DNA diagnostics. Expert Rev. Mol. Diagn. 5 (4), 477-478 (guest editorial).

40. Demidov VV, Dokholyan NV, Witte-Hoffmann C, Chalasani P, Yiu H-W, Ding F, Yu Y, Cantor CR and Broude NE (2006) Fast complementation of split fluorescent protein triggered by DNA hybridization. Proc. Natl. Acad. Sci. USA 103: 2052-2056 (in the news #17; see below).

41. Demidov VV and Broude NE (2006) Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro. Nature Protocols 1(2), 714-719

BOOK CHAPTERS

1. Demidov, V.V., Erukhimovich I.Y. and Kogan, B.S. (1990) Selective interactions of microbial systems with metals on the river-sea barrier. In: Theory and Practice of Modern Exploration Geochemistry, Nauka, Moscow, 243-247.

2. Demidov, V.V., Kogan, B.S. and Kosovets, Yu.G. (1992) Genetic monitoring of pollutants in spatial ecogeochemical models. In: Mathematical Models in Exploration Geochemistry, IMGRE, Moscow, 36-43.

3. Demidov, V.V., Frank-Kamenetskii, M.D. and Nielsen, P.E. (1996) Complexes of duplex DNA with homopyrimidine peptide nucleic acid (PNA): new principle of biomolecular recognition. In: Biological Structure and Dynamics, Sarma, R.H. & Sarma, M.H. (Eds.), Adenine Press, Schenectady NY, v. 2, 129-134.

4. Demidov, V.V. and Frank-Kamenetskii, M.D. (1999) PNA directed genome rare cutting. In P.E. Nielsen and M. Egholm (eds), PNA: Protocols and Applications. Horizon Scientific Press, Wymondham, England.

5. Demidov, V.V., Bukanov, N.O. and Frank-Kamenetskii, M.D. (1999) Duplex DNA capture. In: P.E. Nielsen and M. Egholm (eds), PNA: Protocols and Applications. Horizon Scientific Press, Wymondham, England.

6. Kolotov, B.A., Solomin, G.A., Rubeikin, V.Z., Demidov, V.V., Kalugin, D.E. and Minachëva, M.I. (2002) Hydrogeochemical processes as the basis of interactions of geological substrates affected by anthropogenesis and natural environment using sulfide dumps as an example. In: Applied Geochemistry, v. 2 (Ecological Geochemistry), IMGRE, Moscow, 70-98.

7. Demidov, V.V. and Frank-Kamenetskii, M.D. (2002) PNA openers and their applications. In: P.E. Nielsen (ed), Peptide nucleic acids: Methods and Protocols, Humana Press, 119-130.

8. Frank-Kamenetskii, M.D. and Demidov, V.V. (2004) PNA directed genome rare cutting: new developments. In: P.E. Nielsen (ed), Peptide Nucleic Acids: Protocols & Applications (2nd Edition), chapter 9, pp. 187-205, Horizon Bioscience.

9. Demidov, V.V. (2004) PNA openers for duplex DNA: basic facts, fine tuning and emerging applications. Ibid., chapter 10, pp. 207-226, Horizon Bioscience.

10. Kuhn, H. and Demidov, V.V. (2004) Rolling-circle amplification of duplex DNA sequences assisted by PNA openers. In: V.V. Demidov and N.E. Broude (eds.), DNA Amplification: Current Technologies and Applications, Horizon Bioscience, chapter 3.4, pp. 227-243, Wymondham, England.

11. Demidov, V.V. (2005) Rolling-circle amplification (RCA). In: Encyclopedia of Diagnostic Genomics and Proteomics, J. Fuchs and M. Podda (eds.), Marcel Dekker Press, 1175-1179.

BOOKS

1. Demidov, V.V. and Broude, N.E., Eds. (2004) DNA Amplification: Current Technologies and Applications, Horizon Bioscience, Wymondham, England [reviewed in Drug Discov. Devel. 2004, J Antimicrobial Chemother. 2004, Emerging Infect. Diseases 2005, Expert Rev. Mol. Diagn. 2005, Microbiol. Today 2005]

BOOK REVIEWS

1. Demidov, V.V. (2000) Peptide nucleic acids: protocols & applications. Modern Drug Discovery 3(7/8), 83.

2. Demidov, V.V. (2001) Nucleic acids: structures, properties and functions. Drug Discov. Today 6(4), 178-179.

3. Demidov, V.V. (2001) A history of molecular biology. Modern Drug Discovery 4(3), 73.

4. Demidov, V.V. (2001) Dealing with DNA: DNA technology - the awesome skill. ChemBioChem 2(10), 787.

5. Demidov, V.V. (2002) The misunderstood gene. Modern Drug Discovery 5(7), 63.

RESEARCH NEWS

1. Demidov, V.V. Microbial miners. (1987) Chemistry & Life/Khimiya I Zis'n (December issue; in Russian).

2. Demidov, V.V. (2001) The kiss of cancer. Modern Drug Discovery 4(2), p. 11.

3. Demidov, V.V. (2001) Splice junction function. Modern Drug Discovery 4(2), p. 16.

4. Demidov, V.V. (2001) Shining light on drug incompatibility. Modern Drug Discovery 4(3), p. 11.

5. Demidov, V.V. (2001) Smoking, genotypes, and lung cancer. Modern Drug Discovery 4(3), p. 15.

6. Demidov, V.V. (2001) Photochemicals for periodontitis. Modern Drug Discovery 4(4), p. 11.

7. Demidov, V.V. (2001) Cobra conjugates and cancer. Modern Drug Discovery 4(6), p. 11.

8. Demidov, V.V. (2001) Cleaving RNAs pockets. Modern Drug Discovery 4(6), p. 14.

9. Demidov, V.V. (2001) DNA dumbbells: decoys for gene-regulatory proteins. Drug Discov. Today 6(14), 749-750.

10. Demidov, V.V. (2001) Mass marker for proteomics. Modern Drug Discovery 4(8), p. 11.

11. Demidov, V.V. (2001) Cytomegalovirus, autoimmunity, and lupus. Modern Drug Discovery 4(8), p. 16.

12. Demidov, V.V. (2001) Transition metals brought cellular. Modern Drug Discovery 4(10), p. 12.

13. Demidov, V.V. (2001) Releasing therapy from within. Modern Drug Discovery 4(10), p. 17.

14. Demidov, V.V. (2002) A new use for old stuff: DNA hairpins in DNA amplification. Trends Biotechnol. 20(5), 189-190.

15. Demidov VV (2002) On proteins, DNA and topology. Trends Biotechnol. 20(6), 234.

16. Demidov VV (2002) New task for PNA: installation of transcription factors upstream of genes. Trends Biotechnol. 20(7), 282.

17. Demidov VV (2002) Scoring SNPs by rolling the DNA minicircles. Trends Biotechnol. 20(9), 372.

18. Demidov VV (2002) Invader assay goes on surface. Trends Biotechnol. 20(11), 448.

19. Demidov VV (2002) A happy marriage: advancing DNA polymerases with DNA topoisomerase supplements. Trends Biotechnol. 20(12), 491.

20. Demidov VV (2004) mtDNA instability and mitochondrial disorders. Drug Discov. Today 9(15), 679.

21. Demidov VV (2004) Antisense PNA averts normal splicing. Drug Discov. Today 9(19), 855-856.

22. Demidov VV (2004) Expressing proper glycoproteins in milk. Drug Discov. Today 9(19), 857.

COMMENTARIES/FEATURES

1. Demidov, V.V. (1993) Scientific idea as all-planetary phenomenon. Izvestiya newspaper (April issue; in Russian).

2. Demidov, V.V. (2001) Human genome: how much do we know? Trends Biotechnol. 19(3), 90.

3. Demidov, V.V. (2002) Anthrax-related panic is more dangerous than the disease. Trends Biotechnol. 20(3), 97.

4. Demidov, V.V. (2002) Bioterrorism: how well are we protected? Trends Biotechnol. 20(5), 192.

5. Demidov, V.V. (2003) Tough nuts to crack: encouraging progress in peptide nucleic acid hybridization to structured DNA/RNA targets. Drug Discov. Today 8(9), 390.

6. Demidov, V.V. (2003) Silent chromatin deadly for cancer cells. Drug Discov. Today 8(16), 723-724.

7. Demidov, V.V. (2003) Infection stage clues to new antimalarial medicines . Drug Discov. Today 8(20), 913-915.

8. Demidov V.V. (2004) Proper refolding helps express "difficult" proteins. Drug Discovery & Development 7(6), 41-46.

9. Demidov V.V. (2004) Caught and found in a radio field: advances in ion traps. Drug Discovery & Development 7(7), 39-40.

10. Demidov V.V. (2004) Get it cell-free! Innovative technologies for high-throughput protein expression in vitro. Modern Drug Discov. 2004, 7(9), 61-65.

11. Demidov V.V. (2005) RTS means rapid translation, and it truly is. Biochemica 3, 30-32.

INTERVIEWS

1. Foubister V. (2003) New PNA tool makes great antisense. Drug Discovery Today 8 (12), 520-521.

2. Borman S. (2006) New class of topoisomerase. Chemical & Engineering News 84 (4), 11.

IN THE NEWS [SCI-POP AND GENERAL PERIODICALS ABOUT V.V. DEMIDOV' RESEARCH]

1. Senior, K. (2000) Pseudocomplementary strategy strengthens PNA therapeutic potential. Drug Discovery Today 5 (No 12), 538-540.

2. Sinclair, B. (2000) A thousand points of light: novel fluorescent probes, tags, and molecular beacons. The Scientist 14 (No 20), 19-21.

3. Schwartz, J. (2001) A nano-key unlocks the secrets of DNA. BU Bridge newspaper (Jan. 26), p. 2.

4. Lawrence, R.N. (2001) Artificial primosome for DNA labeling and sequencing. Drug Discovery Today 6 (No 7), 335-336.

5. Harper, C. (2001) Promising primosomes. Modern Drug Discovery 4 (No 5), p. 13.

6. Craig, D.J. (2001) Unlocking DNA's secrets. BU Bridge newspaper (June 8), p. 11.

7. Garner, P. (2001) Novel method of targeting dsDNA with molecular beacons. Expert Rev. Mol. Diagn. 1 (No 3), 245.

8. Jovine, L. (2001) New molecular beacons for targeting double-stranded DNA under native conditions.Trends Biotechnol. 19 (No 12), 487-488.

9. Anonymous (2002) New tricks for molecular beacons. Anal. Chem. 74, (No 7), 183A.

10. Garner, P. (2002) RCA reactions can take place under true topological constraints. Expert Rev. Mol. Diagn. 2 (No 1), 5.

11. Demidov, V.V. (2002) On proteins, DNA and topology. Trends Biotechnol. 20 (No 6), 234.

12. Anonymous (2002) Commentary on a paper by Demidov 'Anthrax-related panic is more dangerous than the disease' in Trends Biotechnol. 20(3), 97. BU Bridge newspaper (March 8), p. 2.

13. Corey, D. (2003) The BioMedNet Commentary on a paper by Demidov and co-workers (Kuhn et al.) 'Artificial Site-Specific DNA-Nicking System Based On Common Restriction Enzymes Assisted by PNA Openers' in Biochemistry 2003 42:4985-4992. (http://update.bmn.com/rsearch/section/record?uid=UPDATE.Corey04062003103).

14. Lauzon, C. (2003) Rare-cleaving PNA-assisted DNA "nickases" introduced. Expert Rev. Mol. Diagn. 3 (No 4), 407.

15. Anonymous (2003) DNA mimics target double stranded DNA. Chem. & Eng. News 81 (No 29), 24.

16. Willis, R.C. (2004) Delivering a nuclear payload. Modern Drug Discovery 7 (No 6), 16.

17. Pines J. (2006) Faculty of 1000 Biology Comment (http://www.f1000biology.com/article/16461889 or www.f1000biology.com/article/16461889/evaluation).

MONOGRAPH AND TEXTBOOKS REFERRED TO V.V. DEMIDOV'S PAPERS

1. Morrison, H., ed. (1990) Bioorganic photochemistry: photochemistry and the nucleic acids (v. 1), Wiley, p. 57.

2. Soyfer, V.N. & Potaman, V.N. (1996) Triple-helical nucleic acids, Springer-Verlag, pp. 148, 270-273.

3. Frank-Kamenetskii, M.D. (1997) Unraveling DNA: the most important molecule of life, Perseus Books, pp. 186-187.

4. Cantor, C.R. & Smith, C.L. (1999) Genomics: the science and technology behind the human genome project, John Wiley & Sons (Wiley-Interscience), pp. 491, 525.

5. Metzler, D.E. (2001) Biochemistry: The chemical reactions of living cells (v. 1), Academic Press.