Broude Natalia E.
B.S., M.S. in Organic Chemistry, Moscow State University
Ph.
D. in Organic Chemistry, Institute of Bioorganic Chemistry, Moscow
D.
Sci. in Molecular Biology, Institute of Bioorganic Chemistry, Moscow
Research
Associate Professor/Senior Instructor
Tel:
(617) 358-4367
Fax:
(617) 353-3501
Email: nebroude@bu.edu
Research Interests
Functional genomics, epigenetic regulation of genome activity, genome-wide methods for complex genome studies.
Current Research
My current interests include development of the new approaches to genome-wide comparative assays: multiplex PCR, targeted genomic differential display, genomic methylation profiling. The most recent project is devoted to visualizing RNA in live cells using fluorescent protein complementation (see scheme below).
Schematics of RNA-dependent fluorescent complex formation within the cell
Selected Publications:
Demidov VV and Broude NE. "Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro" Nat. Prot. 1, 714 - 719 (2006). PDF
Demidov, V.V., Dokholyan, N.V., Witte-Hoffmann, C., Chalasani, P., Yiu, H-W., Ding, F., Yu, Y., Cantor, C.R., Broude, N.E. "Fast complementation of split fluorescent protein triggered by DNA hybridization" Proc. Natl. Acad. Sci. USA 103, 2052-2056 (2006). PDF
Smolina IV, Cherny DI, Nietupski RM, Smith JH, Lane DJ, Broude NE, Demidov VV High-density fluorescently-labeled RCA amplicons for DNA diagnostics. Anal. Biochem. 347, 152-155 (2005). PDF
Broude NE. Cancer roadmap. Expert Rev Mol Diagn. 5(2):121-122., 2005.
Broude NE. Molecular beacons and other hairpin probes. Encyclopedia of Diagnostic Genomics and Proteomics, 846-850, 2005. PDF
Zhang L, Cantor CR, Broude NE. GC/AT-content spikes as genomic punctuation marks. Proc Natl Acad Sci U S A 101(48):16855-60, 2004. PDF
Smolina IV, Demidov VV, Cantor CR, Broude NE. Real-Time Monitoring of Branched RCA Reaction with PNA Beacon. Anal Biochem, 335(2):326-9, 2004. PDF
Broude NE, van Heusden S, and Finkers R."High multiplexity PCR based on PCR suppression" in: DNA Amplification: Current Technologies and Applications, V.V. Demidov and N.E. Broude (eds), pp 61-76, Horizon Scientific Press, Wymondham, England (2004). PDF (Book contents) (Book reviews in Emerging Infectious Diseases, Exp. Rev. Mol. Diag., J. Antimicrobial Chemotherapy)
Broude NE, Cantor CR. Neurological diseases and RNA-directed gene regulation: prospects for new diagnostics and therapy. Expert Rev Mol Diagn. 3(3):269-7, 2003.
Broude NE. Differential display in the time of microarrays. Expert Rev Mol Diagn. May;2(3):209-16, 2002.
Broude NE. Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology. Trends Biotechnol. 20(6):249-56, 2002.
Broude NE, Woodward K, Cavallo R, Cantor CR, Englert D. DNA microarrays with stem-loop probes: preparation and applications. Nucleic Acids Res., 29, e92, 2001.
Broude N, Zhang L, Woodward K, Englert D, Cantor C.R. Multiplex allele-specific target amplification based on PCR suppression. Proc Natl Acad Sci U S A. 98, 206-211, 2001.
Broude N, Demidov V, Kuhn H, Gorenstein J, Pulyaeva H, Volkovitsky P, Drukier A, Frank-Kamenetskii M. PNA openers as a tool for direct quantification of specific targets in duplex DNA. J Biomol Structure and Dynamics 17, 237-244, 1999.
Broude N, Storm N, Malpel S, Lukyanov S, Graber L, Sverdlov E, , Smith CL. PCR based genomic and cDNA differential display. Genet Anal Biomol Eng 15, 51-63, 1999.
Lavrentieva I., Broude N., Lebedev Yu., Lukyanov S., Gottesman I.
and Sverdlov E. Genomic differential display of LTRs reveals high level polymorphisms.
FEBS Lett, 443, 341-347, 1999.
PATENT APPLICATIONS
1.
Methods
and compositions pertaining to PD-loops USSN 09/758,735 (with M. Frank-Kamenetskii,
N. Bukanov, V. Demidov, H. Kuhn)
2.
Novel
compositions and methods for carrying out multiple PCR reactions in a single
tube. PCT/US01/15981, 50661-PCT.