Novel CCD-Based Widefield Sectioning Endomicroscope

photo of Dr. MertzJerome Mertz, Ph.D.HiLo_image
Associate Professor – Biomedical Engineering
Boston University – College of Engineering

.

.

Satish Singh, M.D.
Assistant Professor – Medicine
Boston University – School of Medicine
Boston Medical Center – Gastroenterology

.

Confocal fluorescence endomicroscopy is a rapidly proliferating technology that permits in vivo microscopy of cells and mucosal structures during flexible endoscopy. Detection of mucosal diseases including dysplasia, has been reported for GI, endobronchial, bladder, and peritoneal flexible endoscopy. Available commercial systems involve complex optics to scan a field of view to create a 2D optical section by pixels. The team has developed a novel imaging method (referred to as HiLo microscopy) as well as a clinical prototype that allows confocallike out-of-focus fluorescence background rejection without the use of a confocal scanning mechanism or moving parts. The prototype is specifically designed for clinical applications and performs real-time imaging (10Hz) through an imaging fiber bundle.

OBJECTIVE: Twofold:  1) A validation in a clinical setting of the utility of HiLo endomicroscopy, a novel imaging method that allows confocal-like out-of-focus fluorescence background rejection without the use of a confocal scanning mechanism or moving parts. The prototype is specifically designed for clinical applications and performs real-time imaging (10Hz) through an imaging fiber bundle. 2.) The development of an ultra-high-resolution fluorescence endomicroscope that enables real-time, in vivo imaging of mucosal structures using virtually any endoscope-compatible CCD camera. The low cost of goods, simplicity and versatility of the HiLo endomicroscope will be attractive next to the performance of competing systems.

coulter_CCD coulter_CCD2

Biomicroscopy Lab