Irina V. Smolina, Ph.D.
Research Assistant Professor, Biomedical Engineering
B.S., Moscow Institute of Physics and Technology, Moscow, Russia
M.Sc., Moscow Institute of Physics and Technology, Moscow, Russia
Ph.D., Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia
Office Phone: 617-353-8490, Lab Phone: 617-353-8492
Office: 36 Cummington Street, Room 204; Office hours: By appointment
The development of novel sensitive and selective techniques for molecular diagnostics; exploring the possible use of biological and synthetic DNA analogs for applications in bioengineering, molecular imaging and single-molecule analysis; new methodologies for multiplexed detection and target quantification.
Dr. Smolina’s research is focused on experimental studies of mechanisms and interactions of Peptide Nucleic Acid (PNA), an artificial DNA mimic, with nucleic acids; various PNA-DNA structures and their relations to DNA functioning in the cell. Her current interests include the development of novel approaches to sequence-specific recognition of duplex DNA using PNA molecules. She and her co-workers developing an approach to extend FISH analyses to detect very short DNA sequences on the morphologically preserved whole cell level. The objective is to launch a radically new technology for rapid, ultraspecific and very sensitive detection of short unique sequences (20-30 nucleotides) within genomic DNA in its native, double-stranded form. She is also interested in developing very sensitive system to monitor the expression of specific cell markers. They demonstrated that by merging the single-molecule detection power of the Rolling Circle Amplification (RCA) reaction with highly specific antigen-antibody recognition process the identification of specific low-abundance protein markers can be achieved.
Selected Recent Publications
Konry T, Smolina I, Yarmush JM, Irimia D and Yarmush ML. “Ultrasensitive detection of low-abundance surface-marker protein using isothermal Rolling Circle Amplification in a microfluidic nanoliter platform” Small. (in press, 2011)
Smolina IV, Miller NS and Frank-Kamenetskii MD. “PNA-based microbial pathogen identification and resistance marker detection: an accurate, isothermal rapid assay based on genome-specific features” Artif DNA PNA XNA. 1(2), 1-7. (2010)
Smolina IV, Kuhn H, Lee C and Frank-Kamenetskii MD. “Fluorescence-based detection of short DNA sequences under non-denaturing conditions” Bioorg. Med. Chem. 16(1), 84-93. (2008)
Smolina IV, Lee C and Frank-Kamenetskii MD. “Detection of low copy genomic DNA sequences in individual bacterial cells using PNA-assisted rolling circle amplification and fluorescence in situ hybridization” Appl. Environ. Microbiol. 73(7), 2324-2328. (2007)
Smolina IV, Demidov VV, Soldatenkov VA, Chasovskikh SG and Frank-Kamenetskii MD. “End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes” Nucleic Acids Res. 33, e146. (2005)
Smolina IV, Cherny DI, Nietupski RM, Beals T, Smith JH, Lane DJ, Broude NE and Demidov VV. “High-density labeled fluorescent RCA amplicons for fast and sensitive DNA diagnostics” Anal. Biochem. 347, 152-155. (2005)
Smolina IV, Demidov VV, Cantor CR and Broude NE. “Real-time monitoring of branched rolling-circle DNA amplification (RCA) with peptide nucleic acid (PNA) beacon” Anal. Biochem. 335(2), 326-329. (2004)
Smolina IV and Demidov VV. “Sequence-universal recognition of duplex DNA by oligonucleotides via pseudocomplementarity and helix invasion” Chem. Biol. 10, 591-595. (2003)
Smolina IV, Demidov VV and Frank-Kamenetskii MD. “Pausing of DNA polymerase on duplex DNA template due to ligand binding” J. Mol. Biol. 326(4), 1113-1125.(2003)