Dan Ehrlich, Ph.D.
Research Professor, Biomedical Engineering
Ph.D., Optics, University of Rochester
Complete Publication List
Cell Photometrics Laboratory
Office Phone: 617-358-2919
Lab Phone: 617-358-4976
Office: ERB 247; Office hours: By appointment
Lab: ERB 216
New instrumentation and methods for cell-based assays, deep-UV microscopy, microfluidics for assay of DNA, RNA and protein.
The emerging view is that cancer shares an analogy to contagious disease. Under healthy systemic control, or with the intervention of drugs, an evolving balance is developed between healthy and potentially malignant rare progenitor cell types. Therefore, “base-line” genomic data is insufficient, and averaged genomic data or averaged expression patterns are very indirect and blunt as a diagnostic.
Our laboratory is developing the new methods and instruments needed to gather sufficiently detailed molecular snapshots from sufficiently specific rare-cell phenotypes for both drug development and clinical diagnosis. To some large part, the technology that can fill the gap can be assembled from high-speed microscopy and microfluidics, however, the instruments and work flow need to be redesigned, and other elements such as efficient cost-effective quantitative expression analysis need to be re-thought in format.
Selected Recent Publications
McKenna B, Evans J, Cheung M, and Ehrlich DJ. “A Parallel Microfluidic Flow Cytometer for High-Content Screening” Nature Methods. DOI: 10.1038/nmeth.1595 (Published online April 10, 2011)
Ehrlich, DJ, McKenna, BK, Evans, JG, Belkina, AC, Denis, GV, Sherr, DH and Cheung MC. “Parallel Imaging Microfluidic Cytometer” Methods in Cell Biology. 102, 49-75. (2011)
Cheung, MC, Evans, JG, McKenna, BK, and Ehrlich, DJ. “Deep Ultraviolet Mapping of Intracellular Protein and Nucleic Acid in Femtograms per Pixel” Cytometry Part A. 79A, 920-932. (2011)
Ueberfeld J, Mckenna B, Ramdhanie K, and Ehrlich DJ. “Microdevice DNA Forensics” Humana Press. (2009)
Lin A, Hosoi A, and Ehrlich DJ. “Vertical Hydrodynamic Focusing in Microchannels” Biomicrofluidics. 3: 014101-014112. (2009)
Mckenna BK, Salim H, Bringhurst FR and Ehrlich DJ. “384-Channel Parallel Microfluidic Cytometer for Rare-Cell Screening” Lab on a Chip. 9: 305-310. (2009)
Ueberfeld J and Ehrlich DJ. “Scaling of Nucleic Acid Assays on Microelectrophoresis Array Devices: High-Dynamic Range Multi-gene Readout from less than 10 Transcripts” Electrophoresis. 30: 2090–2099. (2009)
Ueberfeld J, McKenna B, Rubin-Berjerano I, Verstrepen, K, and Ehrlich DJ.. “Reaction-Mapped Quantitative Multiplexed PCR on a Microfluidic Device” Analytical Chemistry. 80: 7430-7436. (2008)
Zeskind BJ, Jordan CD, Timp W, Trapani L, Waller G, Horodincu V, Ehrlich DJ, and Matusudaira P “Nucleic Acid and Protein Mass Mapping by Live-Cell Deep-Ultraviolet Microscopy” Nature Methods. 4(7): 567-569. (2007)
Mckenna BK, Salim H, Bringhurst FR and Ehrlich DJ “Parallel Microfluidic Cytometer for Rare-Cell Screening” Lab On a Chip. (2008)
Ueberfeld J, El-Difrawy SA, Ramandie K, and Ehrlich DJ. “Solid-Support Sample Loading for DNA Sequencing” Anal. Chem. 78: 3632-3627. (2006)
Goedecke N, McKenna B, El-Difrway S, Gismondi E, Swenson A, Carey L, Matsudaira P, and Ehrlich DJ. “Microdevice DNA Forensics by the Simple Tandem Repeat Method” J Chroma A. 1111: 206-213. (2006)
Aborn JH, El-Difrawy SA, Novotny M, Gismondi EA, Lam R, Matsudaira P, McKenna BK, O’Neil T, Streechon P and Ehrlich DJ. “A 768-lane Microfabricated System for High-Throughput DNA Sequencing” Lab on a Chip. 6: 669-674. (2005)
El-Difrawy SA, Lam R, Aborn JH, Novotny M, Gismondi EA, Matsudaira P, McKenna BK, O’Neil T, Streechon P and Ehrlich DJ. “High Throughput System for DNA Sequencing” Rev Sci Instrum. 76 (7): 074301. (2005)
Callewaert N, Contreras R, Mitnik-Gankin L, Carey L, Matsudaira P and Ehrlich DJ. “Total Serum Protein N-glycome Profiling on a Capillary Electrophoresis-Microfluidics Platform” Electrophoresis. 25 (18-19): 3128-3131. (2004)