Natalia E. Broude, Ph.D.

photo of Dr. Broude

Research Professor, Biomedical Engineering

B.S., M.S. in Organic Chemistry, Moscow State University
Ph. D. in Organic Chemistry, Institute of Bioorganic Chemistry, Moscow
D. Sci. in Molecular Biology, Institute of Bioorganic Chemistry, Moscow
Phone: (617) 358-4367
Fax: (617) 353-3501
Office: 44 Cummington Street, Room 622
Office hours: By appointment

Research Interests

Functional genomics, genome-wide methods for genome studies, bacterial internal organization.

Current Research

Professor Broude’s group is working on the development of methods of RNA labeling in bacterial cells.

Selected Recent Publications

Toran P, Smolina I, Driscoll H, Ding F, Sun Y, Cantor CR, Broude NE. “Labeling native bacterial RNA in live cells” Cell Res. (2014 Apr 15) doi: 10.1038/cr.2014.47.

Broude NE. “Analysis of RNA localization and metabolism in single live bacterial cells: achievements and challenges” Mol Microbiol. (2011 Apr 1) doi: 10.1111/j.1365-2958.2011.07652.x. [Epub ahead of print]

Borogovac A, Broude NE. “Visualization of induced RNA in single bacterial cells” Methods Mol Biol. 714: 189-199. (2011)

Yiu HW, Demidov VV, Toran P, Cantor CR, Broude NE. “RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides Pharmaceuticals” 4(3), 494-508. (2011) doi:10.3390/ph4030494

Borogovac A, Broude NE. “Imaging of induced RNA in live cells” RNA visualization: Methods in Mol Biol. Humana Press. (2010)

Valencia-Burton M, Shah A, Sutin J, Borogovac A, McCullough RM, Cantor CR, Meller A, Broude NE. “Spatiotemporal patterns and transcription kinetics of induced RNA in single bacterial cells” Proc Natl Acad Sci U S A. 106 (38): 16399-16404. (2009)

Ding F, Sharma S, Chalasani P, Demidov VV, Broude NE, Dokholyan NV. “Large Scale Simulations of 3D RNA folding by Discrete Molecular Dynamics: From Structure Prediction to Folding Mechanisms, RNA. 14(6): 1164-1173. (2008)

Valencia-Burton MA, Broude NE. “Visualization of RNA using fluorescence protein complementation triggered by aptamer-protein interactions (RFAP) in live bacterial cells” Curr Protoc Cell Biol. Chapter 17: Unit 17.11. (2007)

Valencia-Burton MA, McCullough RM, Cantor CR, Broude NE. “RNA visualization in live bacterial cell using split fluorescent protein approach” Nat Methods. 4(5): 421-427. (2007)

Demidov VV, Broude NE. “Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitroNature Prot. 1: 714 – 719. (2006)

Demidov VV, Dokholyan NV, Witte-Hoffmann C, Chalasani P, Yiu H-W, Ding F, Yu Y, Cantor CR, Broude NE. “Fast complementation of split fluorescent protein triggered by DNA hybridization” Proc Natl Acad Sci U S A. 103(7): 2052-2056. (2006)

Smolina IV, Cherny DI, Nietupski RM, Smith JH, Lane DJ, Broude NE, Demidov VV. “High-density fluorescently-labeled RCA amplicons for DNA diagnostics” Anal. Biochem. 347(1): 152-5. (2005)

Broude NE. “Cancer roadmap” Expert Rev Mol Diagn. 5(2): 121-122. (2005)

Broude NE. “Molecular beacons and other hairpin probes” Encyclopedia of Diagnostic Genomics and Proteomics. 846-850. (2005)

Zhang L, Cantor CR, Broude NE. “GC/AT-content spikes as genomic punctuation marks” Proc Natl Acad Sci U S A. 101(48): 16855-60. (2004)