BME PhD Prospectus Defense - Nga Ho

Starts:
3:00 pm on Monday, December 10, 2012
Ends:
5:00 pm on Monday, December 10, 2012
Location:
ERB 705, 44 Cummington St
Committee Members:
Dr. Mario Cabodi, Department of Biomedical Engineering (Advisor)
Dr. Catherine Klapperich, Department of Biomedical Engineering (Advisor)
Dr. James Galagan, Department of Biomedical Engineering
Dr. Ahmad Khalil, Department of Biomedical Engineering
Dr. Nira Pollock, Beth Israel Deaconess Medical Center

Title: "A Point-of-Care Multiplexed Virus Detection Platform for HIV, Hepatitis B, and Hepatitis C from Patient Samples to Semi-Quantitative Answers"

Abstract:
There are 35 million people that get infected with HIV all over the world and more than 1 million in the U.S. A significant population of HIV patients is co-infected with hepatitis B (HBV) and/or hepatitis C (HCV) while most of them do not know about the co-infection. If known early, the patient can get medication for both HIV and HBV at the same time or specific treatment for HCV according to the HIV status and progression. However, for determining/modifying the treatment, the viral load for each virus and its genotypes must be known. At the moment, there are no multiplexed viral load tests for HIV, HBV, and HCV. Therefore, we proposed to build a diagnostic platform that will detect and quantify HIV, HBV, and HCV from patient's plasma. The platform will contain three parts: sample enrichment, sample RNA/DNA extraction, and multiplex detection. For sample enrichment, a dialysis/concentration chip has been developed to concentrate human plasma up to a factor of 10 based on water evaporation. For sample RNA/DNA extraction, a polymer composite solid-phase-extraction (SPE) column in syringe needle has been built. For multiplex detection, a simple microfluidic system is built to automatically load sample targets and nucleic acid amplification reagents to different reaction chambers without cross-contamination between samples. The whole platform will be rapid, robust, portable, inexpensive, multiplexed, quantitative, highly specific, and sensitive. Also, it will detect directly the viral load of HIV, HBV and HCV genotypes in a close and continuous fashion.