shepro@bu.edu

Cell Signaling and Gene Regulation

Professor of Biology
Ph.D., Boston University, 1959

Microvascular research; biology of endothelial cells and pericytes; inflammatory edema

The long-term objective of my laboratory is to delineate the post-translational cellular events that regulate capillary junctional permeability and the control of inflammatory edema. The mechanism in part involves changes and movement of cytoskeletal proteins as determined by 2D gel electrophoresis and isoelectric focusing, immunoblotting, and immunochemistry. The membrane-cytoskeletal changes are in response to second messengers mobilized by local hormones secreted by the capillary wall cells (endothelium and pericytes). The control of transport of macromolecules and diapedesis (the migration of cells across the microvascular wall), hence can therefore be achieved by modulating this complex cellular mechanism. Experimental protocols are restricted to using monolayers of endothelial cells in co-culture with pericytes.

Lim MJ, Chiang ET, Hechtman HB, Shepro D. 2001. Inflammation-induced subcellular redistribution of VE-cadherin, actin, and gamma-catenin in cultured human lung microvessel endothelial cells. Microvasc Res. Nov;62(3):366-82.

Wang Q, Chiang ET, Lim M, Lai J, Rogers R, Janmey PA, Shepro D, Doerschuk CM. 2001. . Changes in the biomechanical properties of neutrophils and endothelial cells during adhesion. Blood. Feb 1;97(3):660-8.

Chiang ET, Lim MJ, Patton WF, Shepro D. 2000. NFkappaB translocation in human microvessel endothelial cells using a four-compartment subcellular protein redistribution assay. J Biochem Biophys Methods. Nov 20;46(1-2):53-68.

Shojaee N, Patton WF, Hechtman HB, Shepro D. 1999. Myosin translocation in retinal pericytes during free-radical induced apoptosis. J Cell Biochem. Oct 1;75(1):118-29.

Lee CS, Patton WF, Chung-Welch N, Chiang ET, Spofford KH, Shepro D. 1998. Selective propagation of retinal pericytes in mixed microvascular cell cultures using L-leucine-methyl ester. Biotechniques. Sep;25(3):482-8, 490-2, 494.

Lim MJ, Patton WF, Lopez MF, Spofford KH, Shojaee N, and Shepro D. 1997. A luminescent europium complex for the sensitive detection of proteins and nucleic acids immobilized on membrane supports. Analyt. Biochem. 245:184-195.

Wang Q, Patton WF, Hechtman HB, and Shepro D. 1997. A novel anti-inflammatory peptide inhibits endothelial cell cytoskeletal rearrangement, nitric oxide synthase translocation, and paracellular permeability increases. J. Cell. Physiol. 172:171-182.

Hastie LE, Patton WF, Hechtman HB, and Shepro D. 1997. H2O2-induced filamin redistribution in endothelial cells is modulated by the cyclic AMP-dependent protein kinase pathway. J. Cell. Physiol. 172:373-381.

Hastie LE, Patton WF, Hechtman HB, and Shepro D. 1997. Filamin distribution in an endothelial cell reoxygenation injury model. Free Radical Biol. And Med. 20: 955-966.

Wang Q, Patton WF, Chiang ET, Hechtman HB and Shepro D. 1996. Filamin translocation is an early endothelial cell inflammatory response to brodykinin; regulation by calcium, protein kinases, and protein phosphastases. J. Cell. Biochem. 62: 383-396.

Questions and comments are always welcome.
Copyright © 1996, The Trustees of Boston University
This document was last modified on October 14, 2003.