BME Core facilities logoBU College of Engineering logoBME logo
Design element - photo Design element - vertical color gradient

Micro/Nano Imaging (MNI) Facility

Lab director: Dr. Phil Allen
email: mnibme@bu.edu
Location: ERB 501/509 [5th floor of 44 Cummington Street, Boston MA]
   

The Micro and Nano Imaging (MNI) Facility suite is a central teaching and research facility with a focus on imaging biological materials ranging from tissues to individual molecules. Instrumentation includes Confocal, TIRF and multiple Widefield systems with the ability to maintain cells and tissues under appropriate conditions. We’re located on the 5th floor of 44 Cummington Street, Boston MA.

Training and General Policies

Click here to view a detailed list of our training and general policies

Hardware:

Olympus FV1000 scanning confocal microscope

Olympus FV1000 scanning confocal microscope with simscan accessory and 405, 454, 488, 515, 543 and 633nm excitation lines.
- 3 Fluorescence detectors, two with spectral selection using diffraction gratings and adjustable slits for wavelength selection between 400 and 750nm with 2nm resolution.
- Second scan head (simscan) allows concurrent photo-activation/photo-bleach and imaging for high temporal precision studies of diffusion and movement.

- 1 transmission detector.

- Temperature controlled stage incubator provides stable conditions for imaging over ~24 hours.
- Capable of XYZT and lambda scans.

Olympus IX81ZDC with TIRF Illumination

Olympus IX81ZDC with TIRF Illumination.
- 488, 543 and 633 nm excitation in TIRF and epifluorescence.
- Roper Cascade 512b EM camera for fast sensitive detection.

- Hardware autofocus.
- IPlab software for hardware control, image acquisition and analysis.

-Temperature controlled stage incubator provides stable conditions for imaging over ~24 hours.

Olympus IX81 photo-activation system

Olympus IX81 photo-activation system.

- Fast filter wheel control for ratiometric imaging of green (fluorecein) and red (rhodamine) dyes.
- WF dual excitation system for photoconversion studies.

-Temperature controlled stage incubator provides stable conditions for imaging over ~24 hours.

- Computer controlled sequential phase contrast and fluorescence imaging.
- Roper coolsnap-HQ scientific grade CCD camera.
- IPlab software for hardware control, image acquisition and analysis.

Olympus IX81 ratio imaging system

Olympus IX81 ratio imaging system.
- Fast filter wheel control of excitation and emission wavelengths.
- Ideal for FRET & ratiometric dye studies.

- Current filters allow imaging of DAPI, Fura-2 (340/380 ex), Fluorescein, Rhodamine, Cyan Fluorescent Protein, Green Fluorescent Protein, Yellow Fluorescent Protein.
- Dual view accessory for no-delay CFP-YFP FRET.
- Roper coolsnap-HQ scientific grade CCD camera.

-Temperature controlled stage incubator provides stable conditions for imaging over ~24 hours.

- Computer controlled sequential phase contrast and fluorescence imaging.
- IPlab software for hardware control, image acquisition and analysis.

Olympus IX81 color imaging system

Olympus IX81 color imaging system.
- DIC, Phase contrast, Fluorescence imaging.
- Q-Imaging LCD color camera for rapid sequential red/green/blue high resolution color imaging.

-Temperature controlled stage incubator provides stable conditions for imaging over ~24 hours.
- IPlab software for hardware control, image acquisition and analysis.

Services:

© 2008 BME Core Facilities. All Rights Reserved.

BU Department of Biomedical Engineering Boston University