Rederivation

Biosecurity of our animal colonies is a top priority at BU.  The entry of pathogens into our facilities can go undetected but may result in lost time, money, and effort. To protect the integrity of everyone’s research, there are updated health status standards for each of our animal colonies.  Our goal is to provide healthy animals that can be used in research so you can perform your work with complete trust in your results.

Animal biosecurity is the product of all actions undertaken to prevent the introduction of disease agents into a specific area or colony.  While we continue to make improvement s in our facilities and our practices, we still have repeated incidence of pathogens being detected in different colonies.  To help shore up our program, we have worked closely with the Mouse Users Advisory Committee to refine our excluded pathogen program with the goal of establishing a minimally acceptable health status in all mice.  The intent is to maintain consistency between the health status of mice received from approved vendors so their health status remains the same from entry into the facility until the completion of your research.

While we have many of the pieces in place to exclude pathogens from infecting our mice, we still do not have the internal consistency we need to support your research.  To help improve the situation, we have developed a collaboration with the Transgenic (Tg) Core Lab to provide a large scale rederivation service for the medical campus rodent researchers.

The intent of the program is to rederive as many lines as possible in the shortest amount of time.  We will begin with our cleanest facilities, the 670 Albany Street rodent barrier, and work our way through the W9 barrier and then the W8 conventional facility.  Our plan is to optimize our likelihood for success, try to derive each line no more than twice, and get as many lines cleaned up as we can.   Not every line will get rederived to start, but it will allow us to move through as many lines as we can economically and efficiently.

By creating these economies of scale and some other efficiencies, we can offer this program at a substantially reduced cost.  By grouping the work and the testing, we can offer this program at the cost of $250-$300 per line.   This is a limited time offer, so when the program ends, the cost will revert back to the current estimates of roughly $1300.00 per line.

The program should have other benefits you might find helpful.  Having a common health status among different colonies will provide for better utilization of existing facilities and should allow for reduced crowding of rooms particularly in the conventional 8th floor facility.  More importantly, we believe we will need less Personal Protective Equipment (PPE) to maintain our colonies.  Consequently, we expect that the cost to operate the barrier or sterile facilities will become less than the conventional facility.  While the per diem costs are now the same at $1.01 per cage per day, we expect to see the cost of the conventional caging to rise in the next fiscal year to about $1.06 per cage per day while we see no change in the cost of the barrier or sterile cage per diems.

To enter the program, please look for more information below.  There you will find program information about optimizing the success of your rederivation as well as the entry form to sign up.  Our goal is for you to provide us with the mice to be rederived and we return to you a pathogen free line in 3-4 months time.  Please feel free to contact me at drjlevin@bu.edu if you have any questions about the program or its goals.

 

Request for Rederivation

Please provide all of the information below if you are interested in the LASC Rederivation Program. Please note this is not for quarantine rederivation. Thank you.

 

General guidelines on number of mice needed for rederivation

Type/Strain

Age

(min-max)

Males

(min-max)

Females

(min-max)

Excellent Breeders(proven, and line produce litters of 6+ pups) Males: 2-9 mo. Female: 3-10 wk 3-5 4-5
Average Breeders(not proven, line produces < 6 pups per litter) Males: 2-7  mo. Female: 3-10 wk 3-5 6-10
C57Bl6 background Female: 3-8  wk Males 2-7 mo. (< 6 preferred) 3-5 Depends on breeding efficiency of line (excellent or average)
FVB background 5-10 wks (7 weeks preferred)        Males:2-9 mo (<6 preferred) 3-5 Depends on breeding efficiency of line (excellent or average)
Heterozygous Lines 2-10 mo.(<6 months is preferred) 3 males 6-8 Females (can be purchased; age is strain dependant)
Note:  Males being used should be set up with females 2 weeks prior to rederivation for 3-5 days, and then housed without female for 5-7 days before rederivation.

 

DAY

PROCESS STEP

GROUP

Day -14 Mice are transferred to rederivation protocol.                                     Set up with females for 3-5 days LASC
Day -7 Females removed from males LASC
Day 1 Prepare PMS Injections                                                                     Provide  PMS Injections Transcore LASC
Day 3 Prepare HCG Injections                                                                      Provide HCG Injections Transcore LASC
Set up donor breeder pairs.                                                                       Set up recipient females with vasectomized males Make up M2 medium for culture and wash steps LASC           Transcore Transcore
Day 4 Plug Checks on donors females.                                                                   Plug checks on recipient  females LASC            Transcore
Oviduct Collection                                                                                  Make transfer needles and anesthetics for rederivation LASC            Transcore
Wash and delivery to Transcore                                                    Isolate  and wash  embryos 5 times before implantation LASC             Transcore
Implantation Surgery  & recovery Transcore
Day 5-25 Daily Husbandry                                                                                         Post-op monitoring and analgesia LASC           Transcore
Day 25 Birth of Litter LASC
Day 26-46 Daily Husbandry LASC
Day 46 Wean litter and transfer to LASC quarantine room LASC
Day 47-74 Daily Husbandry LASC
Day 74 Collect and send out serology/fecal samples LASC
Day 75-84 Daily Husbandry LASC
Day 84 Read sample results and release from quarantine LASC