PPE Upgrades

Effective November 20, 2006

Dear BUMC animal researchers

Please find below the details on the revised Personal Protective Equipment (PPE) for all animal housing and use areas in the W, R, and M Buildings, 670 Albany St 8th floor animal facilities.

These PPE changes are being made as part of the ongoing LASC operations changes and to improve animal biosecurity for all facilities.

These changes go into effect immediately. We have updated signage and supplies at all entry points in these facilities. Entry points are demarcated by red and white checkered tape on the floors.

Please address any questions to the LASC staff who are available to assist research staff members with the changes, or please call the LASC main office at 8-4086 and ask to speak to a facility manager, supervisor or veterinary staff.

Thank you for your adherence to these changes and your contribution to improved animal health in the BUMC LASC animal care and use areas.

Personal Protective Equipment SOP

I. Purpose

This Standard Operating Procedure defines the requirements for personal protective equipment (PPE) and protective clothing. Functions:

  • Protect personnel who enter animal facilities and/or perform animal procedures from exposure to animal dander, hair, secretions and excretions that may cause allergic respiratory and skin responses, or that may cause illness.
  • Prevent exposure (to animal dander, hair, secretions, and excretions) of persons by contact with persons who have been in animal facilities or have conducted animal procedures.
  • Minimize the risk of possible ocular, oral or dermal exposure to chemical disinfectants.
  • Minimize the transmission of disease agents among animals.
  • To protect personnel working with macaques or macaque tissues from the risk of Herpes B virus infection and other naturally occurring infectious diseases of macaques.
II. Scope/Responsibility

This procedure applies to anyone entering an animal or procedure room occupied by one or more animals.

III. Equipment/Materials
  • Scrub suit – This is clothing, typically referred to as “scrubs,” worn in place of street clothing when handling potentially contaminated, and/or mildly messy, materials to avoid contamination of street clothing. Usually scrubs consist of lightweight, loose fitting, cotton shirt and pants. Usually scrubs are provided and laundered by the institution.
  • Cloth lab coat -Cotton fabric coat that covers torso and arms, usually worn over street clothing. Laboratory coats minimize contamination of clothing and skin beneath the gown by particulates and mists. Should be removed once contaminated.
  • Disposable lab coat – This is lightweight fabric that covers the torso and arms, usually worn over scrubs or street clothing. Laboratory coats minimize contamination of clothing and skin beneath the gown by particulates and mists. Should be removed once contaminated.
  • Face Mask – A variety of masks are used in the animal facilities. For the purpose of this SOP a face mask refers to a surgical mask or cone mask. Face mask and eye splash shield combination : This protects the user’s mouth and eyes from potential splashes or particulates.
  • Bouffant Cap – This protects the wearer’s hair from particulate contamination and minimizes contamination of the environment by the wearer.
  • Disposable exam gloves – Gloves typically made of latex, nitrile, synthetic material, or vinyl and protect the wearer’s hands from contamination. Exam gloves are important in minimizing contamination from animal to animal or from area to area, provided that they are removed and replaced appropriately.
  • Shoe covers – Lightweight fabric or plastic booties worn over shoes to prevent contamination of the shoes and to prevent contamination of the environment by material that may be on the shoes. Shoe covers are especially important in minimizing spread of contamination from area to area, provided that they are removed and replaced appropriately.
  • Waterproof shoe covers: These are booties worn over shoes that are resistant to water.
  • Face shield: This is eye protection that provides splash protection from the sides and top. The top portion of the shield is composed of soft foam that rests against the wearer’s forehead creating a protective barrier.
  • Safety glasses: Hard plastic eye protection is worn to protect the wearer’s eyes from splashes and particulates.
  • Tyvek ® or equivalent jumpsuit: This is made of lightweight and water resistant fabric that covers legs, torso and arms.
  • Sleeve covers: These are lightweight and water resistant fabric that covers the lower arms.
  • Rubber boots: These are boots made from lightweight rubber that are waterproof.
  • N-95 Respirator: This is a respirator whose filtering efficiency has been determined to be at least 95% for the most penetrating sized particle (~0.3 um). These minimize exposure to rodent dander and allergens. They are available to those who choose to use them. Respiratory fit testing by EHS is required for use.
IV. Procedures

A. ALL ANIMAL AREAS

  1. The following must be worn when entering the animals corridors on W8, R8 and M9:
  2. Cloth lab coat
  • The following must be worn when entering the animal corridors on W7, W9:
  • Disposable lab coat
  • Shoe covers
  • These items must be removed as you exit across the thresholds demarcated by the checkered strip on the floors.

B. RODENT ANIMAL AREAS

  1. The following must be worn to enter the W9 Barrier Area rodent housing rooms:
  2. Disposable lab coat
  3. Face Shield or safety glasses
  4. Face Mask (or face mask & face shield combination)
  5. Shoe covers
  6. Gloves – these must be changed between racks (if handling animals)
  7. Sleeve covers (if handling animals). These must be changed between racks.
  8. Bouffant Cap
  • The following must be worn to enter the W9 Barrier Area rodent procedure rooms:
  • Disposable lab coat
  • Shoe covers
  • Bouffant Cap
  • Face Shield or safety glasses (if handling animals)
  • Face Mask (if handling animals)
  • Gloves (if handling animals)
  • Sleeve covers (if handling animals)
  • The following must be worn to enter W8, R8, and M9 rodent housing rooms:
  • Cloth lab coat
  • Face Shield or safety glasses
  • Face Mask (or face mask & face shield combination)
  • Gloves – these must be changed between racks (if handling animals)
  • Sleeve covers (if handling animals). These must be changed between racks.
  • The following must be worn to enter any rodent procedure rooms:
  • Cloth lab coat
  • Face Shield or safety glasses (if handling animals)
  • Face Mask (if handling animals)
  • Gloves (if handling animals)
  • Sleeve covers (if handling animals).
  • The following must be worn to enter the 670 Albany Street Rodent Barrier Facility:
  • surgical scrub suit
  • coveralls
  • shoecovers
  • bouffant cap
  • gloves
  • The following must be worn to enter 670 Albany Street Rodent Barrier Facility animal housing rooms in addition to item 5a-e:
  • Face shield or safety glasses
  • Face mask
  • Sleeve covers (if handling animals)

C. MACAQUE ANIMAL AREAS

  1. The following must be worn by LASC staff or research staff to enter a room housing or holding a macaque:
  2. Scrubs and a disposable lab coat or Tyvek or equivalent jumpsuit if wearing street clothes
  3. Bouffant Cap
  4. Waterproof shoe covers – shoe covers are removed upon leaving the room.
  5. Face Shield ( the mask-face shield combination is not acceptable ). The face shield must provide splash protection from the sides and top.
  6. Face Mask
  7. 2 pairs of gloves
  8. Sleeve covers
  • The following must be worn by LASC staff during cage change in a macaque holding room :
  • Scrubs and a disposable Tyvek or equivalent jumpsuit.
  • Bouffant Cap
  • Waterproof Shoe covers– shoe covers are removed upon leaving room.
  • Face Shield ( the mask-face shield combination is not acceptable ). The face shield must provide splash protection from the sides and top.
  • Face Mask
  • 2 pairs of gloves
  • Sleeve covers

D. CAT, CHINCHILLA, RABBIT, PIG ANIMAL AREAS

  1. The following must be worn to enter a room housing or holding a cat, chinchilla, rabbit or pig or a procedure room to handle or observe the animals:
    1. Cloth or Disposable lab coat
    2. Shoe covers (not required in cat areas)
    3. Gloves
  2. The following must be worn to enter a room housing cat, chinchilla, rabbit or pig to perform physical work (such as washing pens):
    1. Disposable lab coat
    2. Waterproof shoe covers/rubber boots (not required in cat areas)
    3. Gloves
    4. Face shield

E. CAGE WASH AREAS

  1. The following must be worn to enter the clean side of the cage wash:
    1. Scrubs
    2. Disposable lab coat
    3. Bouffant Cap
    4. Shoe covers
    5. Face Shield/safety glasses
    6. Gloves
  2. The following must be worn to enter the dirty side of the cage wash :
    1. Scrubs
    2. Disposable lab coat
    3. Bouffant Cap
    4. Face shield/safety glasses
    5. Face mask (or face mask and splash shield combination)
    6. Rubber boots/waterproof shoe covers
    7. Gloves