Techniques in Molecular Biology

GMS BT 413

Prereq: one semester of biology, two semesters of chemistry and BT 110 (Intro Biomed Lab Sci). The course is comprised of a lecture part focusing on the structure, function, and analysis of DNA and RNA and a laboratory component in which students isolate, analyze, and manipulate DNA and RNA in the test tube. Experiments include the isolation of genomic DNA and RNA, followed by their analysis through gel electrophoresis, spectrophotometry, PCR, and blotting techniques. Students are introduced to recombinant DNA technology through bacterial transformation, plasmid preps, mapping of plasmids by restriction digests, and purification of recombinant protein from bacteria by column chromatography. The course also includes a bioinformatics component in which students use a web platform to access various genetic databases, retrieve genetic sequence information and analyze the sequences using free software for translation and restriction enzyme mapping. Laboratory course.

FALL 2014 Schedule

Section Instructor Location Schedule Notes
A1 Doerre R 5:30 pm-8:30 pm 72 East Concord
T: R 1016 &
R: L 210
Prereqs: Bt 110
or equivalent
GMS BT342
Gns BT405
MED Campus
A1 Doerre T 5:30 pm-8:30 pm 72 East Concord
T: R 1016 &
R: L 210
Prereqs: Bt 110
or equivalent
GMS BT342
Gns BT405
MED Campus

Note that this information may change at any time. Please visit the Student Link for the most up-to-date course information.