Single Molecule Approaches for Biophysics and Bioengineering - Fundamentals and Applications
ENG BE 705
The emergence of single-molecule (SM) methods in biology and bioengineering in the past decade have revolutionized the way scientists approach the molecular biology of the cell. The ability to directly probe biomolecular process in real- time, in their native cellular environment, revealed the mechanism of fundamental processes in biology with unprecedented detail and accuracy. As SM methods are refined they are increasingly recruited by bioengineers to invent the future platforms for molecular diagnostics and analytical detection. This course covers experimental methods for investigating the molecular machinery of a living cell in vitro and in live cells, and novel tools for sensing biomolecules and their application in biotechnology. Fundamental principles underlying fluorescence of single molecules, force measurements of biomolecules, ion channel kinetics, and stochastic sensing, will be covered in the context of relevant biological and biotechnological examples. There will be an emphasis on fundamental physical concepts underlying these systems, coming from statistical mechanics of soft matter. 4 cr.