% This file contains the flow cytometry data for the publication % "Combinatorial promoter design for engineering noisy gene expression" % by K. F. Murphy, G. Balazsi and J. J. Collins (2006). % % Three experiment sets were performed for all strains. % The experiment set for each strain is indicated by the number after "_" % % The names of the strains are: % % BG: native promoter, no GFP (autofluorescence background) % WT: native promoter upstream from GFP (no TetR sites, wild type) % S1: one TetR site close to the TATA box (position 1) % S2: one TetR site in intermediate position (position 2) % S3: one TetR site close to the transcription START site (position 3) % D12: two TetR sites in positions 1 and 2, respectively % D13: two TetR sites in positions 1 and 3, respectively % D23: two TetR sites in positions 2 and 3, respectively % T123: three TetR sites in positions 1, 2 and 3, respectively BG_1= 1.0e+002 *[... 0 0.02353909332672 0.00901965721401 0.00383177766825 0.50000000000000 0.02434910957971 0.00850698461655 0.00349375593744 1.50000000000000 0.02687216192776 0.00783734886569 0.00291653082724 2.50000000000000 0.02839413341901 0.00825026493874 0.00290562307960]; BG_2= 1.0e+002 *[... 0 0.02502519324545 0.00833945095761 0.00333242220183 0.50000000000000 0.02527793129348 0.00732078128135 0.00289611566562 1.50000000000000 0.02757828354245 0.00794532693064 0.00288100850019 2.50000000000000 0.03040604415602 0.00839319704294 0.00276037126036]; BG_3= 1.0e+002 *[... 0 0.02613976398127 0.00773215535302 0.00295800503729 0.50000000000000 0.02673162353037 0.00724613608700 0.00271069809088 1.50000000000000 0.02819490832471 0.00798718016442 0.00283284487839 2.50000000000000 0.03201645272832 0.00858130470102 0.00268027965929]; WT_1= 1.0e+003 *[... 0 1.46762368251610 0.17774707059888 0.00012111215751 0.05000000000000 1.47003106620002 0.17879505341277 0.00012162671764 0.15000000000000 1.44705482705175 0.17846111307764 0.00012332712606 0.25000000000000 1.46911628686690 0.19221188441628 0.00013083503745]; WT_2= 1.0e+003 *[... 0 1.46985285556290 0.23660203719160 0.00016096987960 0.05000000000000 1.44505564671962 0.19325005557624 0.00013373191269 0.15000000000000 1.43467050773823 0.20350697862919 0.00014184928005 0.25000000000000 1.44352307344876 0.19627201016357 0.00013596735222]; WT_3= 1.0e+003 *[... 0 1.42904747577561 0.21647760354436 0.00015148384306 0.05000000000000 1.43076426954597 0.23043234056914 0.00016105542015 0.15000000000000 1.44806394882397 0.26513225619891 0.00018309430078 0.25000000000000 1.54105228910317 0.17511870091814 0.00011363579429]; S1_1 = 1.0e+003 *[... 0 0.02147086513156 0.00403156071994 0.00018776889963 0.01000000000000 0.03100271297646 0.00541635970742 0.00017470599143 0.02000000000000 0.05163051985661 0.02868605345353 0.00055560264613 0.02500000000000 0.11509063227815 0.09985384267787 0.00086761051444 0.03000000000000 0.24967734344247 0.20598543929032 0.00082500653223 0.03500000000000 0.67488699741377 0.33019273440559 0.00048925632835 0.04000000000000 1.23927140010813 0.23465732648944 0.00018935103842 0.04500000000000 1.34357388629239 0.20210857245920 0.00015042609455 0.05000000000000 1.34919609377631 0.19770763047599 0.00014653735761 0.05500000000000 1.40396463697658 0.17996245126214 0.00012818161264 0.06000000000000 1.40684496694223 0.20659488207056 0.00014684978582 0.06500000000000 1.40776440943279 0.19438646263176 0.00013808167143 0.07000000000000 1.44569260553949 0.18829183382610 0.00013024334018 0.07500000000000 1.52448400503093 0.21726955740740 0.00014252006363 0.25000000000000 1.49008847864127 0.18898242988391 0.00012682631441]; S1_2 = 1.0e+003 *[... 0 0.02056567198930 0.00373196477805 0.00018146573474 0.01000000000000 0.03087737985304 0.00569696118374 0.00018450273990 0.02000000000000 0.05847435111777 0.03021812711521 0.00051677575788 0.02500000000000 0.14631890901494 0.13476652573520 0.00092104654581 0.03000000000000 0.23335969108000 0.20693757634399 0.00088677515550 0.03500000000000 0.71553466862336 0.33710299367235 0.00047112042009 0.04000000000000 1.29111417765497 0.26067035753451 0.00020189566658 0.04500000000000 1.34790945654613 0.18116294255954 0.00013440290198 0.05000000000000 1.31137489379899 0.22037593720074 0.00016804953202 0.05500000000000 1.30569293026160 0.19451621589988 0.00014897546842 0.06000000000000 1.33298984934846 0.19183749921131 0.00014391519883 0.06500000000000 1.33539987497702 0.17167448324436 0.00012855661174 0.07000000000000 1.40200610501416 0.18912064867634 0.00013489288527 0.07500000000000 1.41489181730010 0.19528810779110 0.00013802334949 0.25000000000000 1.44637172391377 0.18189025472561 0.00012575622969]; S1_3 = 1.0e+003 *[... 0 0.02143820149165 0.00411585367366 0.00019198689196 0.01000000000000 0.02992647324212 0.00521190814689 0.00017415711182 0.02000000000000 0.05506836321716 0.04105935886068 0.00074560703209 0.02500000000000 0.13135876085888 0.12095070972934 0.00092076622022 0.03000000000000 0.23432518994368 0.19958682643467 0.00085175147615 0.03500000000000 0.70180247225867 0.33718019796078 0.00048044886031 0.04000000000000 1.17286100778136 0.24752749489595 0.00021104588971 0.04500000000000 1.33090301915599 0.17634026219485 0.00013249670311 0.05000000000000 1.29545865816608 0.20907841168982 0.00016139334928 0.05500000000000 1.38712165119652 0.16837677678475 0.00012138573184 0.06000000000000 1.38139507623744 0.19647382155686 0.00014222855209 0.06500000000000 1.41346915221159 0.16070732144263 0.00011369708436 0.07000000000000 1.42169508557552 0.22083638957451 0.00015533315956 0.07500000000000 1.43350435349150 0.18698400572254 0.00013043839404 0.25000000000000 1.46018681942589 0.18075537553431 0.00012378921185]; S2_1 = 1.0e+002 *[... 0 0.53007985970853 0.08727543678995 0.00164645826834 0.10000000000000 0.66836004515994 0.10638228952209 0.00159169133901 0.20000000000000 0.93368759934489 0.20253588147525 0.00216920393521 0.25000000000000 1.34816322984195 0.48145398192726 0.00357118464048 0.30000000000000 2.17759395358377 0.89206636479584 0.00409656889122 0.35000000000000 3.47583683412381 1.33943639094768 0.00385356521284 0.40000000000000 4.81152801393607 1.56013588288531 0.00324249568612 0.45000000000000 6.01078455149593 1.56041229653313 0.00259602100718 0.50000000000000 8.67333274517454 1.58528717663006 0.00182777165734 0.55000000000000 7.79642753308267 1.44085850225366 0.00184810093615 0.60000000000000 8.32082876450366 1.28442166032727 0.00154362227211 0.65000000000000 8.66455462502203 1.25150050407789 0.00144439103709 0.70000000000000 8.62191530981919 1.30346648940078 0.00151180618524 0.75000000000000 8.86012669261948 1.26659609156380 0.00142954625312 2.50000000000000 8.70075395159891 1.35223033902059 0.00155415306138]; S2_2 = 1.0e+002 *[... 0 0.49429198914217 0.08161480699765 0.00165114565460 0.10000000000000 0.62098567666224 0.10235484978210 0.00164826426162 0.20000000000000 0.92442045035347 0.20006061757893 0.00216417342890 0.25000000000000 1.57149512487297 0.61486329630351 0.00391260072381 0.30000000000000 2.00820710445152 0.80788527371411 0.00402291811399 0.35000000000000 3.08557370866845 1.22658501887349 0.00397522514347 0.40000000000000 5.46035881263484 1.50928685607331 0.00276407999522 0.45000000000000 5.92090538935416 1.50897700019656 0.00254855786568 0.50000000000000 6.86447315575979 1.43781754330837 0.00209457814268 0.55000000000000 7.88327668396199 1.27367928371442 0.00161567243518 0.60000000000000 7.99428120789689 1.36729912730431 0.00171034654867 0.65000000000000 8.61685779396953 1.20017266371185 0.00139281939241 0.70000000000000 8.46811395582688 1.22911789064715 0.00145146593097 0.75000000000000 8.35340121531978 1.19559634526681 0.00143126891005 2.50000000000000 8.61641552378992 1.24747911787330 0.00144779359170]; S2_3 = 1.0e+002 *[... 0 0.49801408366265 0.15759439789807 0.00316445665028 0.10000000000000 0.61594373260423 0.09557736475419 0.00155172233590 0.20000000000000 0.86408445419528 0.17540871714615 0.00202999505771 0.25000000000000 1.37599937829670 0.45572233400404 0.00331193706329 0.30000000000000 1.84714946249986 0.74428136706828 0.00402935107407 0.35000000000000 2.62833809701632 1.08102299271611 0.00411295256856 0.40000000000000 4.51855499105638 1.50895421908057 0.00333946189007 0.45000000000000 5.89934574228619 1.54223778611998 0.00261425224676 0.50000000000000 6.93724183446366 1.40543180600702 0.00202592303907 0.55000000000000 8.51207420695977 1.26042405628226 0.00148074843527 0.60000000000000 7.99674368631884 1.25909928619826 0.00157451499709 0.65000000000000 8.19965361336367 1.30266554440180 0.00158868362717 0.70000000000000 8.57094791389160 1.24408179850201 0.00145151016084 0.75000000000000 8.26486407684104 1.17290321580985 0.00141914398701 2.50000000000000 8.51155029976951 1.27071240586267 0.00149292709449]; S3_1 = 1.0e+003 *[... 0 0.66239170746957 0.08863851590842 0.00013381585987 0.01000000000000 0.87630071964847 0.11123196969155 0.00012693355968 0.02000000000000 1.11802465552046 0.13686011641353 0.00012241243137 0.02500000000000 1.31768733014938 0.16305644819494 0.00012374441528 0.03000000000000 1.47866569516394 0.19397317975143 0.00013118122669 0.03500000000000 1.61435741372348 0.20681819543263 0.00012811177604 0.04000000000000 1.66572508925006 0.21572331747510 0.00012950715509 0.04500000000000 1.65115851537785 0.23285488420010 0.00014102515418 0.05000000000000 1.73879764006530 0.22959953466367 0.00013204500016 0.05500000000000 1.72014264616346 0.22528350903449 0.00013096792265 0.06000000000000 1.72855263794833 0.23568761630814 0.00013634968999 0.06500000000000 1.71256924536615 0.22386769788567 0.00013072037729 0.07000000000000 1.81889178814951 0.23817188639813 0.00013094340628 0.07500000000000 1.89064512100292 0.23365217952344 0.00012358330864 0.25000000000000 1.68278958097333 0.23451932959043 0.00013936343096]; S3_2 = 1.0e+003 *[... 0 0.61773057954213 0.08163294895136 0.00013214976181 0.01000000000000 0.82316888906384 0.10108300567226 0.00012279740770 0.02000000000000 1.08446276804959 0.12647743719090 0.00011662681368 0.02500000000000 1.43616440567986 0.18215278550771 0.00012683282275 0.03000000000000 1.48515767632391 0.19504194227261 0.00013132743101 0.03500000000000 1.54405697613157 0.19724589454429 0.00012774521769 0.04000000000000 1.80421174999052 0.19408589876901 0.00010757379159 0.04500000000000 1.62169727390184 0.23180882651983 0.00014294210778 0.05000000000000 1.86601187646298 0.19541714809945 0.00010472449322 0.05500000000000 1.70253614196712 0.21642898136949 0.00012712151950 0.06000000000000 1.66968485886873 0.22645727020997 0.00013562874994 0.06500000000000 1.69347462887116 0.21928732377523 0.00012948958315 0.07000000000000 1.66229408729420 0.22341618086435 0.00013440231940 0.07500000000000 1.64465275604632 0.21850155153116 0.00013285573549 0.25000000000000 1.68601030467981 0.22196923523138 0.00013165354602]; S3_3 = 1.0e+003 *[... 0 0.63264863369290 0.07758857649658 0.00012264086630 0.01000000000000 0.87554601902842 0.10478964127288 0.00011968490404 0.02000000000000 1.05671824493101 0.12482833185493 0.00011812830189 0.02500000000000 1.37833513200852 0.17378126759322 0.00012608056166 0.03000000000000 1.42523032875114 0.17498883184105 0.00012277933490 0.03500000000000 1.50136375779914 0.18880142538307 0.00012575328557 0.04000000000000 1.49706049389019 0.19699139703415 0.00013158546220 0.04500000000000 1.53983047076646 0.22618292420007 0.00014688819873 0.05000000000000 1.40066177079080 0.21378027505220 0.00015262805019 0.05500000000000 1.45231581907864 0.21983506279529 0.00015136863477 0.06000000000000 1.57083448625789 0.22492013232638 0.00014318512504 0.06500000000000 1.55781725845466 0.21942557321484 0.00014085450140 0.07000000000000 1.65323166907850 0.20824830522682 0.00012596438184 0.07500000000000 1.63114191262821 0.22558124379799 0.00013829651611 0.25000000000000 1.62653496715129 0.20961828091949 0.00012887413130]; D12_1 = 1.0e+003 *[... 0 0.00628570120558 0.00131826279035 0.00020972406216 0.01000000000000 0.00669050631978 0.00136535165517 0.00020407299387 0.02000000000000 0.00774158973470 0.00633254801092 0.00081799064894 0.02500000000000 0.01009080620918 0.00828188084201 0.00082073529809 0.03000000000000 0.02350957641539 0.04035373091485 0.00171648056102 0.03500000000000 0.15847998926032 0.15898406585198 0.00100318069552 0.04000000000000 0.39494080653183 0.23222481335125 0.00058799903558 0.04500000000000 0.69055453703755 0.22156695546730 0.00032085366699 0.05000000000000 0.82508775953142 0.17643705606712 0.00021384035096 0.05500000000000 0.95390405796916 0.15616999099177 0.00016371666489 0.06000000000000 0.97499419725973 0.15144517178357 0.00015532930576 0.06500000000000 1.02667391658056 0.14340204626038 0.00013967633145 0.07000000000000 1.10119147932815 0.13930478860223 0.00012650369279 0.07500000000000 1.03629533461700 0.14861824979432 0.00014341302603 0.25000000000000 1.07575899614206 0.15482934813470 0.00014392568288]; D12_2 = 1.0e+003 *[... 0 0.00628663744048 0.00134020369060 0.00021318291428 0.01000000000000 0.00657181867778 0.00137285175639 0.00020889982267 0.02000000000000 0.00747379721522 0.00248149394648 0.00033202585982 0.02500000000000 0.01332811575810 0.02220530452856 0.00166604979515 0.03000000000000 0.02108418330464 0.03528863858839 0.00167370194418 0.03500000000000 0.18979308234432 0.17416006282578 0.00091763124701 0.04000000000000 0.42073895627588 0.25582606277261 0.00060803987593 0.04500000000000 0.64926446901395 0.22234417587021 0.00034245548075 0.05000000000000 0.83795769421277 0.17169977768352 0.00020490268049 0.05500000000000 0.93077307907991 0.14747511013329 0.00015844367811 0.06000000000000 0.98146353060519 0.14354296163612 0.00014625399433 0.06500000000000 1.00845755594653 0.14212717151866 0.00014093520414 0.07000000000000 1.00340188881774 0.14204950388953 0.00014156790561 0.07500000000000 0.99352319636888 0.14080379286207 0.00014172169646 0.25000000000000 1.13792616376318 0.13639680587280 0.00011986437277]; 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D13_1 = 1.0e+003 *[... 0 0.01801858883987 0.00364090731511 0.00020206395448 0.01000000000000 0.02831209878496 0.00567047776636 0.00020028461364 0.02000000000000 0.05010140588380 0.02847044454231 0.00056825640000 0.02500000000000 0.12867551946057 0.12052610573944 0.00093666694523 0.03000000000000 0.32513526392375 0.24003427812404 0.00073825974835 0.03500000000000 1.05404874456852 0.32690665733242 0.00031014377562 0.04000000000000 1.48625089923585 0.27956995201496 0.00018810414322 0.04500000000000 1.55949051069284 0.22868489629893 0.00014664077449 0.05000000000000 1.71842714861844 0.23893998934225 0.00013904574863 0.05500000000000 1.67608291435164 0.25058831161556 0.00014950830264 0.06000000000000 1.65158530625398 0.23595108251765 0.00014286339411 0.06500000000000 1.64907813563959 0.22292640763277 0.00013518244091 0.25000000000000 1.67448194137693 0.24027915384002 0.00014349462237]; D13_2 = 1.0e+003 *[... 0 0.01954726843038 0.00380287397681 0.00019454759064 0.01000000000000 0.02566902323581 0.01151789523452 0.00044870796714 0.02000000000000 0.04986296578280 0.03027583247013 0.00060718074015 0.02500000000000 0.12940803938268 0.11523542419609 0.00089048118452 0.03000000000000 0.30291526244498 0.23882739417000 0.00078842971543 0.03500000000000 1.03024227186783 0.32771176977411 0.00031809194664 0.04000000000000 1.33262110267782 0.25353568164894 0.00019025338946 0.04500000000000 1.45235658073761 0.25655001898147 0.00017664396085 0.05000000000000 1.56606139725670 0.21407755817212 0.00013669806212 0.05500000000000 1.51067391478893 0.19716876548978 0.00013051709145 0.06000000000000 1.59571212452991 0.23089329378808 0.00014469608286 0.06500000000000 1.47111751395341 0.22174797200787 0.00015073437024 0.25000000000000 1.61542889187650 0.17737837206585 0.00010980264929]; 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T123_2 = 1.0e+003 *[... 0 0.00355671480196 0.00093850468667 0.00026386841198 0.01000000000000 0.00464207425966 0.00142916950978 0.00030787303904 0.02000000000000 0.01623114112424 0.03089005213761 0.00190313496144 0.02500000000000 0.06215686053909 0.08450184903053 0.00135949351846 0.03000000000000 0.23631118317696 0.21240496172545 0.00089883584378 0.03500000000000 0.50475383847172 0.29366969986514 0.00058180775951 0.04000000000000 0.71139321694741 0.30933643638435 0.00043483186094 0.04500000000000 1.10343944534494 0.28758616063914 0.00026062704379 0.05000000000000 1.15113935006867 0.23098946432378 0.00020066160045 0.05500000000000 1.21668872571644 0.20475783044327 0.00016829105597 0.06000000000000 1.25389748406857 0.19173161912444 0.00015290852846 0.06500000000000 1.28369527815115 0.18936635964388 0.00014751659749 0.07000000000000 1.29264490465181 0.18238623214232 0.00014109538628 0.07500000000000 1.30590544984331 0.18909456090445 0.00014479958019 0.25000000000000 1.33942504716858 0.19151109928152 0.00014298007917]; T123_3 = 1.0e+003 *[... 0 0.00367845428581 0.00096231304435 0.00026160799335 0.01000000000000 0.00439179725953 0.00110584650000 0.00025179816705 0.02000000000000 0.01274222479503 0.02390808501458 0.00187628812073 0.02500000000000 0.05936577493127 0.08299433454775 0.00139801652794 0.03000000000000 0.21953028893970 0.19675000810857 0.00089623171845 0.03500000000000 0.48142603566371 0.27753956488871 0.00057649471430 0.04000000000000 0.72570563322434 0.30880639283892 0.00042552569348 0.04500000000000 0.95243985881912 0.27305067725692 0.00028668547912 0.05000000000000 1.07022469759710 0.22375241418194 0.00020907050144 0.05500000000000 1.15030986317765 0.22111825795885 0.00019222495176 0.06000000000000 1.22062703923865 0.19084048770114 0.00015634627250 0.06500000000000 1.26110075629239 0.19329532690873 0.00015327508603 0.07000000000000 1.25815615867164 0.19236927191562 0.00015289777075 0.07500000000000 1.28744809220408 0.20070282372011 0.00015589197338 0.25000000000000 1.30491605312650 0.18533828927123 0.00014203081403];